PRDM5 Antibody (YA4517)(PBS only)

Cat. No.: HY-P84820A: Data Sheet User Guide for Antibodies
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PRDM5 Antibody (YA4517) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRDM5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PRDM5 Antibody (YA4517) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRDM5.

Host

Mouse

Molecular Weight

Predicted band size: 73 kDa;

Observed band size: 70-75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9NQX1

Gene ID

11107 [NCBI]

Immunogen

Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May cause G2/M arrest and apoptosis in cancer cells

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues

Isoforms & Post-Translational Modification：Q9NQX1 has 4 isomers: Q9NQX1-1: 73090 Da (predicted); Q9NQX1-2: 69862 Da (predicted); Q9NQX1-3: 12874 Da (predicted); Q9NQX1-4: 58124 Da (predicted).

Subunit：Interacts with EHMT2/G9A, GFI1 and HDAC1

Synonyms

BCS2; PFM2

Documentation

User Guide for Antibodies (1077 KB)