Copy Link Print Information

FGFR1 Oncogene Partner Antibody (YA440)

(Synonyms: FGFR1OP, FOP, CEP43, Centrosomal protein 43, FGFR1 oncogene partner)
Customer Review

Based on 1 Customer Validation

FGFR1 Oncogene Partner Antibody (YA440) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR1 Oncogene Partner.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • FGFR1 Oncogene Partner Antibody (YA440) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500 1:400

Product Details

Description

FGFR1 Oncogene Partner Antibody (YA440) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR1 Oncogene Partner.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human FGFR1 Oncogene Partner.AA range:350-399.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102481

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
    Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane5(40μg) ) using FGFR1 Oncogene Partner(HY-P80396) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80396, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), 293T (lane 4), and MCF-7 (lane 5) using FGFR1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 500) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    FGFR1 Oncogene Partner is required for anchoring microtubules to the centrosomes. Required for ciliation[1][2][3].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cytoplasm, cytoskeleton, cilium basal body

  • Expression


    Tissue_specificity:Ubiquitous. Highly expressed in heart, liver, muscle, kidney, intestine, colon, adrenal gland, prostate, testis, and pancreas

  • Subunit

    Homodimer (PubMed:16690081). Part of a ternary complex that contains CEP350, CEP43 and MAPRE1. Interacts directly with CEP350 and MAPRE1 (PubMed:16314388). Interacts with CEP19 (PubMed:28428259, PubMed:28625565, PubMed:28659385). Interacts (via N-terminus) with CEP350 (via C-terminus) (PubMed:28428259, PubMed:28625565)

  • SwissProt ID

    O95684

  • Gene ID
    11116 [NCBI]
  • Synonyms

    FGFR1OP, FOP, CEP43, Centrosomal protein 43, FGFR1 oncogene partner

  • Research Field

    Tags & Cell Markers

FGFR1 Oncogene Partner Antibody (YA440) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane5(40μg) ) using FGFR1 Oncogene Partner(HY-P80396) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80396, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), 293T (lane 4), and MCF-7 (lane 5) using FGFR1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 500) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FGFR1 Oncogene Partner Antibody (YA440) Product Image
    1/16
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • FGFR1 Oncogene Partner Antibody (YA440) Product Image
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane5(40μg) ) using FGFR1 Oncogene Partner(HY-P80396) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80396, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), 293T (lane 4), and MCF-7 (lane 5) using FGFR1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 500) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • Experimental Validation Results for FGFR1 Oncogene Partner Antibody (YA440)
  • FGFR1 Oncogene Partner Antibody (YA440) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane5(40μg) ) using FGFR1 Oncogene Partner(HY-P80396) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80396, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • FGFR1 Oncogene Partner Antibody (YA440) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), 293T (lane 4), and MCF-7 (lane 5) using FGFR1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1: 500) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • FGFR1 Oncogene Partner Antibody (YA440) Product Image WB
  • FGFR1 Oncogene Partner Antibody (YA440) Product Image WB