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ZO-1/TJP1 Antibody (YA3430)

(Synonyms: ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein; )
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

ZO-1/TJP1 Antibody (YA3430) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZO-1/TJP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL ICC IHC-P
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • ZO-1/TJP1 Antibody (YA3430) Product Image
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Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) ZO-1/TJP1 Antibody (YA3430)

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1 Publications Citing Use of MCE ZO-1/TJP1 Antibody (YA3430) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:100 1:200

Product Details

Description

ZO-1/TJP1 Antibody (YA3430) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZO-1/TJP1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 191kDa
Species Reactivity Database
Immunogen

Recombinant protein within human ZO1 aa 1,401-1,748.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3676581

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    Immunocytochemistry analysis of MCF-7 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    Immunocytochemistry analysis of A431 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    ZO-1/TJP1 is a TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, tight junction; Cell junction; Cell junction, gap junction; Cell projection, podosome

  • Expression


    Tissue_specificity:Isoforms containing the α subunit are present at most epithelial cell junctions. Short isoforms are present at highly specialized epithelial junctions, such as those of endothelial cells, glomeruli, and seminiferous tubules.
    Positive sample: A431 cell lysate, 293T cell lysate, HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Ra

  • Isoforms & Post-Translational Modification

    Q07157 has 2 isomers: Q07157-1: 195459 Da (predicted); Q07157-2: 186966 Da (predicted).
    Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF) (PubMed:19332538, PubMed:7542259). This response is dependent on an intact actin microfilament system (PubMed:7542259). Dephosphorylated by PTPRJ (PubMed:19332538)

  • Subunit

    Homodimer (PubMed:16737969, PubMed:17928286).

  • SwissProt ID

    Q07157

  • Gene ID
    7082 [NCBI]
  • Synonyms

    ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;

ZO-1/TJP1 Antibody (YA3430) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    ZO-1/TJP1 Antibody (YA3430) purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Sep 15:283:116943. [Abstract]

    Customer Validation
     Western blot assay reveals changes in ZO-1, Occludin protein expression in the colon of chronic lead-exposed mice after butyrate intervention (n=3).

  • ZO-1/TJP1 Antibody (YA3430) Product Image
    1/16
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • ZO-1/TJP1 Antibody (YA3430) Product Image
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • Experimental Validation Results for ZO-1/TJP1 Antibody (YA3430)
  • ZO-1/TJP1 Antibody (YA3430) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ZO-1/TJP1 Antibody (YA3430) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling ZO-1 with ZO-1 Antibody (HY-P83694) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ZO-1 Antibody (HY-P83694)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ZO-1/TJP1 Antibody (YA3430) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ZO-1/TJP1 Antibody (YA3430) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using ZO-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ZO-1/TJP1 Antibody (YA3430) Product Image ICC
  • ZO-1/TJP1 Antibody (YA3430) Product Image ICC
  • ZO-1/TJP1 Antibody (YA3430) Product Image IHC-P
  • ZO-1/TJP1 Antibody (YA3430) Product Image IHC-P