NKX3.1 Antibody (YA4990)

Cat. No.: HY-P85298: Data Sheet User Guide for Antibodies
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NKX3.1 Antibody (YA4990) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to NKX3.1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

NKX3.1 Antibody (YA4990) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to NKX3.1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

분자량

Predicted band size: 26 kDa;

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99801

Gene ID

4824 [NCBI]

Immunogen

A synthesized peptide derived from human Nkx 3.1. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is highly expressed in the prostate and at a lower level in the testes.

Induction:By androgens and, in the LNCaP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen-independent phase

Isoforms & Post-Translational Modification：Q99801 has 5 isomers: Q99801-1: 26350 Da (predicted); Q99801-2: 21099 Da (predicted); Q99801-3: 18423 Da (predicted); Q99801-4: 19049 Da (predicted); Q99801-5: 21626 Da (predicted).
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues

Subunit：Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF (PubMed:11809674). Interacts with WDR77 (PubMed:12972618). Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation (PubMed:18077445). Interacts with FEM1B (By similarity)

Synonyms

NKX3-1; NKX3.1; NKX3A; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A

각종 서류

User Guide for Antibodies (1077 KB)