NKX3.1 Antibody (YA4990)
(Synonyms: NKX3-1; NKX3.1; NKX3A; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A)
Based on 1 Customer Validation
NKX3.1 Antibody (YA4990) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to NKX3.1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, IHC-F, IF-Tissue
-
Reactivity :
Human
-
Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IHC-F
IHC-F: Immunohistochemistry-Frozen
|
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
|
|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 | 1:50-200 | 1:50-200 |
Product Details
NKX3.1 Antibody (YA4990) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to NKX3.1.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 28 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 26 kDa
A synthesized peptide derived from human Nkx 3.1. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
NKX3.1 transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells
-
Subcellular Localization
Nucleus
-
Expression
Tissue_specificity:It is highly expressed in the prostate and at a lower level in the testes.
Induction:By androgens and, in the LNCaP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen-independent phase -
Isoforms & Post-Translational Modification
Q99801 has 5 isomers: Q99801-1: 26350 Da (predicted); Q99801-2: 21099 Da (predicted); Q99801-3: 18423 Da (predicted); Q99801-4: 19049 Da (predicted); Q99801-5: 21626 Da (predicted).
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues -
Subunit
Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF (PubMed:11809674). Interacts with WDR77 (PubMed:12972618). Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation (PubMed:18077445). Interacts with FEM1B (By similarity)
-
SwissProt ID
-
Synonyms
NKX3-1; NKX3.1; NKX3A; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A
Documentation
Powered by Bioz
