MYL9 Antibody (YA5904)
(Synonyms: MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL)MYL9 Antibody (YA5904) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL9.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP, FC, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:50-1:200 | 1:50-1:200 | 1:20-1:100 | 1:50-1:100 |
Product Details
MYL9 Antibody (YA5904) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL9.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 20 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 20 kDa
A synthesized peptide derived from human Myosin light chain 9 : 60-80
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MYL9 is a Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation[1][2].
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Subcellular Localization
Cytoplasm, cytoskeleton; Cytoplasm, cell cortex
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Expression
Tissue_specificity:Smooth muscle tissue and some (but not all) non-muscle cells -
Isoforms & Post-Translational Modification
P24844 has 2 isomers: P24844-1: 19827 Da (predicted); P24844-2: 13866 Da (predicted).
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation. Phosphorylated by DAPK3; DAPK3-mediated phosphorylation is inhibited by LUZP1 (PubMed:38009294) -
Subunit
Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19 (By similarity). Interacts with LUZP1; the interaction results in inhibition of phosphorylation of MYL9 by DAPK3 (PubMed:38009294)
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SwissProt ID
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Synonyms
MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL
Documentation
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