MYL9 Antibody (YA5904)

Cat. No.: HY-P86212: Data Sheet User Guide for Antibodies
FAQs
Technical Support

MYL9 Antibody (YA5904) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MYL9 Antibody (YA5904) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL9.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 20 kDa;

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P24844

Gene ID

10398 [NCBI]

Immunogen

A synthesized peptide derived from human Myosin light chain 9 : 60-80

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1:200
IP IP: Immunoprecipitation	1:20-1:100
FC FC: Flow Cytometry	1:50-1:100

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cell cortex

Expression：
Tissue_specificity:Smooth muscle tissue and some (but not all) non-muscle cells

Isoforms & Post-Translational Modification：P24844 has 2 isomers: P24844-1: 19827 Da (predicted); P24844-2: 13866 Da (predicted).
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation. Phosphorylated by DAPK3; DAPK3-mediated phosphorylation is inhibited by LUZP1 (PubMed:38009294)

Subunit：Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19 (By similarity). Interacts with LUZP1; the interaction results in inhibition of phosphorylation of MYL9 by DAPK3 (PubMed:38009294)

Synonyms

MYL9; MLC2; MRLC1; MYRL2; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2; smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRL

Documentation

User Guide for Antibodies (1077 KB)