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Zinc Alpha 2 Glycoprotein Antibody (YA2103)

(Synonyms: Azgp1; ZA2G; ZAG; ZNGP1)
Customer Review

Based on 1 Customer Validation

Zinc Alpha 2 Glycoprotein Antibody (YA2103) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Zinc Alpha 2 Glycoprotein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:40

Product Details

Description

Zinc Alpha 2 Glycoprotein Antibody (YA2103) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Zinc Alpha 2 Glycoprotein.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Species Reactivity Database

Entrez Gene: 563 Human

SwissProt: P25311 Human

Immunogen

A synthesized peptide derived from human ZA2G / AZGP1 aa51-99/298.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), and Ramos (lane 3) using Zinc Alpha 2 Glycoprotein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human colon cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human stomach cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human breast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    Immunohistochemical analysis of paraffin-embedded human lbreast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Zinc Alpha 2 Glycoprotein stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver

  • Subunit

    Interacts with PIP

  • SwissProt ID

    P25311

  • Gene ID
    563 [NCBI]
  • Synonyms

    Azgp1; ZA2G; ZAG; ZNGP1

  • Research Field

    -

Zinc Alpha 2 Glycoprotein Antibody (YA2103) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), and Ramos (lane 3) using Zinc Alpha 2 Glycoprotein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lbreast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    1/16
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), and Ramos (lane 3) using Zinc Alpha 2 Glycoprotein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lbreast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Experimental Validation Results for Zinc Alpha 2 Glycoprotein Antibody (YA2103)
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), and Ramos (lane 3) using Zinc Alpha 2 Glycoprotein antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bladder cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lbreast cancer using Zinc Alpha 2 Glycoprotein antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82358, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image WB
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P
  • Zinc Alpha 2 Glycoprotein Antibody (YA2103) Product Image IHC-P