PACAP Receptor Antibody (YA9031)
(Synonyms: Pituitary adenylate cyclase-activating polypeptide type I receptor, PAC1 receptor, PAC1R, PACAP type I receptor, PACAP-R-1, PACAP-R1, ADCYAP1R1)PACAP Receptor Antibody (YA9031) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PACAP Receptor.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
PACAP Receptor Antibody (YA9031) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PACAP Receptor.
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Host Mouse
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Species Reactivityhuman
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 51 kDa
OMIM: 102981
A synthesized peptide derived from human PACAP Receptor.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
PACAP Receptor is a G protein-coupled receptor activated by the neuropeptide pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP). Binds both PACAP27 and PACAP38 bioactive peptides. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract[1][2][3][4][5].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta. -
Isoforms & Post-Translational Modification
PACAP Receptor has 5 isoforms, P41586-1: amino acid length is 468, molecular weight is 53314 Da (predicted); P41586-2: amino acid length is 496, molecular weight is 56531 Da (predicted); P41586-3: amino acid length is 447, molecular weight is 51030 Da (predicted); P41586-4: amino acid length is 475, molecular weight is 54248 Da (predicted); P41586-5: amino acid length is 411, molecular weight is 46957 Da (predicted).
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Subunit
Interacts with maxadilan, a vasodilator peptide from Lutzomyia longipalpis saliva; the interaction results in ADCYAP1R1 activation
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SwissProt ID
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Synonyms
Pituitary adenylate cyclase-activating polypeptide type I receptor, PAC1 receptor, PAC1R, PACAP type I receptor, PACAP-R-1, PACAP-R1, ADCYAP1R1
Documentation
References
[1]. Liang YL, et al. Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation. Mol Cell. 2020 Feb 6;77(3):656-668.e5. [Content Brief]
[2]. Wang J, et al. Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism. Cell Res. 2020 May;30(5):436-445. [Content Brief]
[3]. Hu E, et al. Discovery of Selective Pituitary Adenylate Cyclase 1 Receptor (PAC1R) Antagonist Peptides Potent in a Maxadilan/PACAP38-Induced Increase in Blood Flow Pharmacodynamic Model. J Med Chem. 2021 Mar 25;64(6):3427-3438. [Content Brief]
[4]. Xu Y, et al. A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2. Nat Commun. 2022 Apr 27;13(1):2272. [Content Brief]
[5]. Piper SJ, et al. Understanding VPAC receptor family peptide binding and selectivity. Nat Commun. 2022 Nov 16;13(1):7013. [Content Brief]
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