Myosin Light Chain 2 Antibody (YA3749)
(Synonyms: MLC2; CMH10; DKFZp779C0562)Based on 1 Customer Validation
Myosin Light Chain 2 Antibody (YA3749) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myosin Light Chain 2.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
Myosin Light Chain 2 Antibody (YA3749) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myosin Light Chain 2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 19 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 19 kDa
Purified recombinant fragment of MYL2 aa 2-166.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A431(lane 2(20μg), C2C12(lane 3(20μg), 293T(lane 4(20μg), HEK293(lane 5(20μg) using Myosin Light Chain 2 Antibody (HY-P84052). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
Myosin Light Chain 2 is a Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly[1][2].
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Subcellular Localization
Cytoplasm, myofibril, sarcomere, A band
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Expression
Tissue_specificity:High expression in type I myofibrils -
Subunit
Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC (PubMed:11773029)
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SwissProt ID
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Synonyms
MLC2; CMH10; DKFZp779C0562
Documentation
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Data Sheet (258 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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