Sodium Potassium ATPase Antibody (YA3646)

Cat. No.: HY-P83949: Data Sheet User Guide for Antibodies
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Sodium Potassium ATPase Antibody (YA3646) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Sodium Potassium ATPase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Sodium Potassium ATPase Antibody (YA3646) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Sodium Potassium ATPase.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 113 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P05023

Gene ID

476 [NCBI]

Immunogen

Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:29499166, PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Cell membrane, sarcolemma; Multi-pass membrane protein; Cell projection, axon; Melanosome

Isoforms & Post-Translational Modification：P05023 has 4 isomers: P05023-1: 112896 Da (predicted); P05023-2: 74140 Da (predicted); P05023-3: 109550 Da (predicted); P05023-4: 113000 Da (predicted).
Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity)

Subunit：Interacts with regulatory subunit FXYD3 (PubMed:21454534).Binds the HLA class II histocompatibility antigen DR1 (PubMed:1380674). Interacts with SLC35G1 and STIM1 (PubMed:22084111).

Synonyms

CMT2DD; HOMGSMR2

Documentation

User Guide for Antibodies (1077 KB)