BUB1 Antibody (YA1567)
(Synonyms: Bub1; BUB1A; BUB1L; hBUB1)
Based on 1 Customer Validation
BUB1 Antibody (YA1567) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:100 |
Product Details
BUB1 Antibody (YA1567) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 122 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 122 kDa
Entrez Gene: 699 Human ; 12235 Mouse ; 296137 Rat
SwissProt: O43683 Human ; O08901 Mouse ;
OMIM: 620183 Human
A synthesized peptide derived from human Bub1 aa1-200/1085.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg), HEK293T (lane4, 20μg) and U2OS (lane5, 20μg) using BUB1 Antibody (HY-P81822). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
BUB1 is a conserved serine/threonine mitotic checkpoint kinase that accumulates at unattached kinetochores and supports spindle assembly checkpoint signaling, chromosome alignment, and high-fidelity chromosome segregation[1][2]. Mechanistically, BUB1 promotes checkpoint control by helping recruit core checkpoint proteins to kinetochores and by enabling formation of an APC/C^CDC20^ inhibitory signal when chromosomes fail to attach properly to spindle microtubules[2][3]. BUB1 kinase activity also phosphorylates histone H2A at Thr121, thereby supporting Aurora B localization and attachment-error correction during mitosis[4]. In disease-relevant models, abnormal BUB1 expression associates with chromosome missegregation, aneuploidy, and cancer-related phenotypes, while pan-cancer analysis found BUB1 overexpression in many TCGA tumors and higher expression in clinically aggressive molecular subtypes[4][5]. Compared with the related isoform BUBR1/BUB1B, BUB1 acts more prominently as a kinetochore recruitment and signaling scaffold, whereas BUBR1 is a component of the diffusible APC/C^CDC20^ inhibitor[2][3]. For experimental applications, BAY 1816032 inhibits BUB1 kinase signaling and sensitizes tumor cells toward taxanes, ATR inhibitors, and PARP inhibitors, while BAY-320 inhibits cellular BUB1 activity but shows off-target concerns at required concentrations[6][7].
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Subcellular Localization
Nucleus; Chromosome, centromere, kinetochore
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Expression
Tissue_specificity:High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index
Induction:Inhibited by phorbol 12-myristate 13-acetate (PMA) -
Isoforms & Post-Translational Modification
O43683 has 3 isomers: O43683-1: 122375 Da (predicted); O43683-2: 115843 Da (predicted); O43683-3: 120002 Da (predicted).
Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction;Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1 -
Subunit
Interacts with BUB3 and KNL1. Interacts (when phosphorylated) with PLK1. The BUB1-BUB3 complex interacts with MAD1L1
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SwissProt ID
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Synonyms
Bub1; BUB1A; BUB1L; hBUB1
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Research Field
Cell Biology
Documentation
References
[1]. Kim T, et al. Bub1 kinase in the regulation of mitosis. Anim Cells Syst (Seoul). 2021 Feb 17;25(1):1-10. [Content Brief]
[2]. Vleugel M, et al. Dissecting the roles of human BUB1 in the spindle assembly checkpoint. J Cell Sci. 2015 Aug 15;128(16):2975-82. [Content Brief]
[3]. Overlack K, et al. A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint. Elife. 2015 Jan 22;4:e05269. [Content Brief]
[4]. Ricke RM, et al. Bub1 kinase activity drives error correction and mitotic checkpoint control but not tumor suppression. J Cell Biol. 2012 Dec 10;199(6):931-49. [Content Brief]
[5]. Cicirò Y, et al. Expression of the checkpoint kinase BUB1 is a predictor of response to cancer therapies. Sci Rep. 2024 Feb 23;14(1):4461. [Content Brief]
[6]. Siemeister G, et al. Inhibition of BUB1 Kinase by BAY 1816032 Sensitizes Tumor Cells toward Taxanes, ATR, and PARP Inhibitors In Vitro and In Vivo. Clin Cancer Res. 2019 Feb 15;25(4):1404-1414. [Content Brief]
[7]. Amalina I, et al. Inhibitors of the Bub1 spindle assembly checkpoint kinase: synthesis of BAY-320 and comparison with 2OH-BNPP1. R Soc Open Sci. 2021 Dec 15;8(12):210854. [Content Brief]
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