2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. BUB1 Antibody (YA1567)

BUB1 Antibody (YA1567) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

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Description

BUB1 Antibody (YA1567) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BUB1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 122 kDa;
Observed band size: 122 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Bub1 aa1-200/1085.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A (PubMed:35044816). Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis
Subcellular Localization：Nucleus; Chromosome, centromere, kinetochore
Expression：
Tissue_specificity:High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index

Induction:Inhibited by phorbol 12-myristate 13-acetate (PMA)
Isoforms & Post-Translational Modification：O43683 has 3 isomers: O43683-1: 122375 Da (predicted); O43683-2: 115843 Da (predicted); O43683-3: 120002 Da (predicted).
Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction;Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1
Subunit：Interacts with BUB3 and KNL1. Interacts (when phosphorylated) with PLK1. The BUB1-BUB3 complex interacts with MAD1L1
Database

Entrez Gene: 699 Human ; 12235 Mouse ; 296137 Rat

SwissProt: O43683 Human ; O08901 Mouse ;

OMIM: 620183 Human

Research Field

Cell Biology
Synonyms
Bub1; BUB1A; BUB1L; hBUB1
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

BUB1 Antibody (YA1567) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BUB1 Antibody (YA1567)
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HY-P81822
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