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Ephrin B2 Antibody (YA3385)

(Synonyms: Efnb2; ephrin B2; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK5)
Customer Review

Based on 1 Customer Validation

Ephrin B2 Antibody (YA3385) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin B2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Ephrin B2 Antibody (YA3385) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Ephrin B2 Antibody (YA3385) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin B2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Ephrin B2 aa50-100.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105747

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), SK-OV-3 (lane 2, 15 μg), 293 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using Ephrin B2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Ephrin B2 Antibody (HY-P83640) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    Immunocytochemistry analysis of HeLa cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Ephrin B2 cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons; (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell junction, adherens junction

  • Expression


    Tissue_specificity:Lung and kidney

  • Subunit

    Interacts with PDZRN3 (By similarity). Binds to the receptor tyrosine kinases EPHA4, EPHB4 and EPHA3

  • SwissProt ID

    P52799

  • Gene ID
    1948 [NCBI]
  • Synonyms

    Efnb2; ephrin B2; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK5

  • Research Field

    Cardiovascular

Ephrin B2 Antibody (YA3385) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), SK-OV-3 (lane 2, 15 μg), 293 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using Ephrin B2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Ephrin B2 Antibody (HY-P83640) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ephrin B2 Antibody (YA3385) Product Image
    1/16
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Ephrin B2 Antibody (YA3385) Product Image
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), SK-OV-3 (lane 2, 15 μg), 293 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using Ephrin B2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Ephrin B2 Antibody (HY-P83640) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Experimental Validation Results for Ephrin B2 Antibody (YA3385)
  • Ephrin B2 Antibody (YA3385) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), SK-OV-3 (lane 2, 15 μg), 293 (lane 3, 15 μg), Mouse brain (lane 4, 15 μg), and Rat brain (lane 5, 15 μg) using Ephrin B2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Ephrin B2 Antibody (YA3385) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SH-SY5Y(lane 2(20ug) and SH-SY5Y(lane 3(40ug) using Ephrin B2 Antibody (HY-P83640) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Ephrin B2 Antibody (YA3385) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ephrin B2 Antibody (YA3385) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Ephrin B2 with Ephrin B2 Antibody (HY-P83640) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ephrin B2 Antibody ((HY-P83640) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ephrin B2 Antibody (YA3385) Product Image WB
  • Ephrin B2 Antibody (YA3385) Product Image WB
  • Ephrin B2 Antibody (YA3385) Product Image ICC
  • Ephrin B2 Antibody (YA3385) Product Image ICC