S100 A8 Antibody (YA5411)

Cat. No.: HY-P85719: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

S100 A8 Antibody (YA5411) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to S100 A8.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

S100 A8 Antibody (YA5411) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to S100 A8.

Host

Mouse

Clonality

Monoclonal

분자량

Observed band size: 14 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P05109

Gene ID

6279 [NCBI]

Immunogen

Synthesized peptide derived from human S100 A8

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
WB WB: Western Blot	1:1000-2000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH2O2idase. Also participates in regulatory T-cell differentiation together with CD69 (PubMed:26296369). Activates NADPH2O2idase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs tH2O2gh the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner

Subcellular Localization：Secreted; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Peripheral membrane protein

Expression：
Tissue_specificity:Calprotectin (S100A8/9) is primarily expressed in myeloid cells. Except in inflammatory states, its expression is limited to specific stages of myeloid differentiation, as both proteins are expressed in circulating neutrophils and monocytes, but absent in normal tissue macrophages and lymphocytes. Calprotectin is also expressed in the epidermis in cH2O2ic inflammatory states, such as psoriasis and malignancies. Calprotectin concentrations are higher in serum at sites of inflammation or in patients with inflammatory diseases such as rheumatoid arthritis, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, Sjgren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Calprotectin is involved in the formation and deposition of amyloid proteins (called amyloid inclusion bodies) in the aging prostate. It is significantly upregulated in a variety of tumors, including gastric cancer, esophageal cancer, colon cancer, pancreatic cancer, bladder cancer, ovarian cancer, thyroid cancer, breast cancer, and skin cancer.

Induction: (Microbial infection) Expression is highly induced in CD14 (+) monocytes, neutrophils, and developing neutrophils of infected by SARS-COV-2

Subunit：Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A9 known as calprotectin (S100A8/A9).

각종 서류

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]