DCAF13 Antibody (YA6537)

Cat. No.: HY-P86844: Data Sheet COA
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User Guide for Antibodies
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DCAF13 Antibody (YA6537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DCAF13.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DCAF13 Antibody (YA6537) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DCAF13.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 51 kDa;

Observed band size: 49 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NV06

Gene ID

25879 [NCBI]

Immunogen

Recombinant protein within Human DCAF13 aa 1-100 / 445.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200
IP IP: Immunoprecipitation	1-2μg/sample

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). Participates in the 18S rRNA processing in growing oocytes, being essential for oocyte nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) transition (PubMed:30283081)|Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex that plays a key role in embryo preimplantation and is required for normal meiotic cycle progression in oocytes (PubMed:16949367, PubMed:30111536, PubMed:31492966). Acts as a maternal factor that regulates oocyte and zygotic chromatin tightness during maternal to zygotic transition (By similarity). Also involved in the transformation of the endometrium into the decidua, known as decidualization, providing a solid foundation for implantation of blastocysts (PubMed:35932979). Recognizes the histone methyltransferases SUV39H1 and SUV39H2 and directs them to polyubiquitination and proteasomal degradation, which facilitates the H3K9me3 removal and early zygotic gene expression, essential steps for progressive genome reprogramming and the establishment of pluripotency during preimplantation embryonic development (PubMed:30111536). Supports the spindle assembly and chromosome condensation during oocyte meiotic division by targeting the polyubiquitination and degradation of PTEN, a lipid phosphatase that inhibits PI3K pathway as well as oocyte growth and maturation (PubMed:31492966). Targets PMP22 for polyubiquitination and proteasomal degradation (By similarity)

Subcellular Localization：Nucleus, nucleolus

Expression：
Tissue_Specificity: Expressed in the endometrium during decidualization. Expression is down-regulated in preeclampsia decidual tissues

Isoforms & Post-Translational Modification：Q9NV06 has 2 isomers: Q9NV06-1: 51402 Da (predicted); Q9NV06-2: 10919 Da (predicted).

Subunit：Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3 (PubMed:34516797)

Synonyms

DCA13_HUMAN antibody; DCAF13 antibody; DDB1 and CUL4 associated factor 13 antibody; DDB1- and CUL4-associated factor 13 antibody; DKFZP564O0463 antibody; GM83 antibody; HSPC064 antibody; MGC126859 antibody; MGC138247 antibody; WD repeat and SOF domain-containing protein 1 antibody; DCA13_HUMAN antibody; DCAF13 antibody; DDB1 and CUL4 associated factor 13 antibody; DDB1- and CUL4-associated factor 13 antibody; DKFZP564O0463 antibody; GM83 antibody; HSPC064 antibody; MGC126859 antibody; MGC138247 antibody; WD repeat and SOF domain-containing protein 1 antibody; WD repeats and SOF1 domain containing antibody; WDSOF1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)