ALKBH5 Antibody (YA3429)

Cat. No.: HY-P83693: Ficha de datos COA
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ALKBH5 Antibody (YA3429) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH5.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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ALKBH5 Antibody (YA3429) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

ALKBH5 Antibody (YA3429) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALKBH5.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Peso molecular

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q6P6C2

Gene ID

54890 [NCBI]

Immunogen

Recombinant protein within human ALKBH5 aa 51-350 / 394.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:2000

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using ALKBH5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178, PubMed:34048572, PubMed:36944332, PubMed:37257451, PubMed:37369679). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing, translation and export (PubMed:23177736, PubMed:34048572, PubMed:36944332, PubMed:37257451). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (PubMed:37369679, PubMed:37474102). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (PubMed:37474102). Also acts as a negative regulator of T-cell development: inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (By similarity). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (By similarity)

Subcellular Localization：Nucleus speckle

Expression：
Tissue_specificity:Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary

Induction:By hypoxia, directly activated by HIF1A. Expression is regulated by PRMT7

Positive sample: HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, F9 cell lysate, PC-12 cell lysate, rat testis tissue lysate,

Isoforms & Post-Translational Modification：Q6P6C2 has 3 isomers: Q6P6C2-2: 44256 Da (predicted); Q6P6C2-1: 52255 Da (predicted); Q6P6C2-3: 43049 Da (predicted).
Phosphorylated at Ser-87 and Ser-325 in response to reactive oxygen species (ROS), promoting sumoylation and inactivation;Acetylated by KAT8 at Lys-235, promoting interaction with PSPC1, thereby facilitating recognition of N(6)-methyladenosine (m6A) mRNA by ALKBH5 (PubMed:37369679). Deacetylated at Lys-235 by HDAC7 (PubMed:37369679);Sumoylated at Lys-86 and Lys-321 by PIAS4 following phosphorylation at Ser-87 and Ser-325 in response to reactive oxygen species (ROS), inhibiting the RNA demethylase activity (PubMed:34048572, PubMed:37257451). Desumoylated by SENP1; relieving RNA demethylase inhibition, leading to N(6)-methyladenosine-containing mRNAs demethylation (PubMed:34048572, PubMed:37257451);Ubiquitinated at Lys-57 via 'Lys-48'-linked polyubiquitin chain, leading to its degradation by the proteasome (PubMed:37454738). Deubiquitinated at Lys-57 by USP9X, promoting its stabilizazion (PubMed:37454738)

Subunit：Monomer (PubMed:24778178). Interacts with RBM33; promoting desumoylation by SENP1 and recruitment to N(6)-methyladenosine-containing mRNAs (PubMed:37257451). Interacts (when acetylated by KAT8) with PSPC1; interaction facilitates recognition of N(6)-methyladenosine (m6A) mRNA (PubMed:37369679)

RRID

AB_3676580

Database

Entrez Gene: 54890 Human ; 268420 Mouse ; 303193 Rat

SwissProt: Q6P6C2 Human ; Q3TSG4 Mouse ; D3ZKD3 Rat

OMIM: 613303 Human

Synonyms

ABH5,AlkB,OFOXD,OFOXD1,RNA demethylase ALKBH5 antibody

Documentación

Select Batch:

Ficha de datos (259 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ALKBH5 Antibody (YA3429) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ALKBH5 Antibody (YA3429)

ALKBH5 Antibody (YA3429) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

ALKBH5 Antibody (YA3429) Related Classifications

Productos vistos recientemente:

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