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  2. Primary Antibodies
  3. Monoclonal Antibodies Recombinant Antibodies
  4. Collagen X Antibody (YA3502)

Collagen X Antibody (YA3502)

Cat. No.: HY-P81102
COA User Guide for Antibodies Technical Support

Collagen X Antibody (YA3502) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen X.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Collagen X Antibody (YA3502)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

Collagen X Antibody (YA3502) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen X.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight
Predicted band size: 66 kDa;
Observed band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Collagen X aa527-600.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from RAJI(lane 2(40μg), HELA(lane 3(40μg), SIHA(lane 4(40μg),THP-1(lane 5(40μg)using Collagen X Antibody (YA3502). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

Background
Function:Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage
Subcellular Localization:Secreted, extracellular space, extracellular matrix
Subunit:Homotrimer
RRID
Database
Research Field

Cell Biology

Synonyms
COAA1_HUMAN antibody; Col10a 1 antibody; COL10A1 antibody; Collagen alpha 1(X) chain antibody; Collagen alpha-1(X) chain antibody; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody; Collagen type X alpha 1 antibody; Collagen X alpha 1 polypeptide antibody; CollagenX antibody; fa66d11 antibody; fb10c08 antibody; OTTHUMP00000040411 antibody; Procollagen type X alpha 1 antibody; Schmid metaphyseal chondrodysplasia antibody; wu:fa66d11 antibody; wu:fb10c08 antibody;
Documentation

Collagen X Antibody (YA3502) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Collagen X Antibody (YA3502)
Cat. No.:
HY-P81102
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