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Collagen X Antibody (YA3502)

(Synonyms: COAA1_HUMAN antibody; Col10a 1 antibody; COL10A1 antibody; Collagen alpha 1(X) chain antibody; Collagen alpha-1(X) chain antibody; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody; Collagen type X alpha 1 antibody; Collagen X alpha 1 polypeptide antibody; CollagenX antibody; fa66d11 antibody; fb10c08 antibody; OTTHUMP00000040411 antibody; Procollagen type X alpha 1 antibody; Schmid metaphyseal chondrodysplasia antibody; wu:fa66d11 antibody; wu:fb10c08 antibody; )
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Based on 1 publication(s) in Google Scholar

Collagen X Antibody (YA3502) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen X.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Collagen X Antibody (YA3502)
  • Collagen X Antibody (YA3502) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Collagen X Antibody (YA3502) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen X.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Collagen X aa527-600.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103210

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen X Antibody (YA3502)
    Western blot analysis of extracts from RAJI(lane 2(40μg), HELA(lane 3(40μg), SIHA(lane 4(40μg),THP-1(lane 5(40μg)using Collagen X Antibody (YA3502). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

Background

  • Function

    Collagen X (COL10A1) is a non-fibrillar collagen produced by hypertrophic chondrocytes and used as a specific marker of chondrocyte hypertrophy during endochondral ossification[1]. Mechanistically, RUNX2 directly regulates Col10a1 enhancer activity, while Cox-2-containing and RUNX2-centered regulatory complexes further shape hypertrophic chondrocyte-specific expression[2][3]. In disease models, serum type X collagen fragments increase in osteoarthritis and correlate with cartilage degradation and inflammation biomarkers[4]. Compared with COL2A1, which suppresses chondrocyte hypertrophy through integrin β1-SMAD1 interaction, COL10A1 identifies the hypertrophic phenotype rather than the stable cartilage matrix phenotype[5]. Human COL10A1 loss-of-function iPSC models show that collagen X is dispensable for hypertrophic differentiation and endochondral ossification in vitro, supporting its use as a marker and model readout rather than a standalone driver[1]. COL10A1 mutations also cause Schmid metaphyseal chondrodysplasia, linking collagen X biology to growth-plate skeletal disease[6]. For experimental applications, COL10A1 supports studies of chondrocyte hypertrophy, osteoarthritis biomarker development, skeletal dysplasia mechanisms, and cartilage-to-bone transition models[1][4][6].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Subunit

    Homotrimer

  • SwissProt ID

    Q03692

  • Gene ID
    1300 [NCBI]
  • Synonyms

    COAA1_HUMAN antibody; Col10a 1 antibody; COL10A1 antibody; Collagen alpha 1(X) chain antibody; Collagen alpha-1(X) chain antibody; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody; Collagen type X alpha 1 antibody; Collagen X alpha 1 polypeptide antibody; CollagenX antibody; fa66d11 antibody; fb10c08 antibody; OTTHUMP00000040411 antibody; Procollagen type X alpha 1 antibody; Schmid metaphyseal chondrodysplasia antibody; wu:fa66d11 antibody; wu:fb10c08 antibody;

  • Research Field

    Cell Biology

[1]. Kamakura T, et al. Collagen X Is Dispensable for Hypertrophic Differentiation and Endochondral Ossification of Human iPSC-Derived Chondrocytes. JBMR Plus. 2023 Mar 29;7(5):e10737. [Content Brief]

[2]. Li F, et al. Runx2 contributes to murine Col10a1 gene regulation through direct interaction with its cis-enhancer. J Bone Miner Res. 2011 Dec;26(12):2899-910. [Content Brief]

[3]. Gu J, et al. Identification and characterization of the novel Col10a1 regulatory mechanism during chondrocyte hypertrophic differentiation. Cell Death Dis. 2014 Oct 16;5(10):e1469. [Content Brief]

[4]. He Y, et al. Type X collagen levels are elevated in serum from human osteoarthritis patients and associated with biomarkers of cartilage degradation and inflammation. BMC Musculoskelet Disord. 2014 Sep 22;15:309. [Content Brief]

[5]. Lian C, et al. Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction. Bone Res. 2019 Mar 6;7:8. [Content Brief]

[6]. Warman ML, et al. A type X collagen mutation causes Schmid metaphyseal chondrodysplasia. Nat Genet. 1993 Sep;5(1):79-82. [Content Brief]

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Collagen X Antibody (YA3502) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAJI(lane 2(40μg), HELA(lane 3(40μg), SIHA(lane 4(40μg),THP-1(lane 5(40μg)using Collagen X Antibody (YA3502). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • Collagen X Antibody (YA3502) Product Image
    1/16
  • Experimental Validation Results for Collagen X Antibody (YA3502)
  • Collagen X Antibody (YA3502) Product Image
  • Experimental Validation Results for Collagen X Antibody (YA3502)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAJI(lane 2(40μg), HELA(lane 3(40μg), SIHA(lane 4(40μg),THP-1(lane 5(40μg)using Collagen X Antibody (YA3502). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem
  • Experimental Validation Results for Collagen X Antibody (YA3502)
  • Collagen X Antibody (YA3502) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAJI(lane 2(40μg), HELA(lane 3(40μg), SIHA(lane 4(40μg),THP-1(lane 5(40μg)using Collagen X Antibody (YA3502). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight.Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room tem

  • Collagen X Antibody (YA3502) Product Image WB