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NF2 Antibody (YA1386)

(Synonyms: ACN; BANF; Merlin; Moesin ezrin radixin like protein; Neurofibromatosis 2; Neurofibromin 2; Nf2; SCH; Schwannomerlin)
Customer Review

Based on 1 Customer Validation

NF2 Antibody (YA1386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NF2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • NF2 Antibody (YA1386) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

NF2 Antibody (YA1386) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NF2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human NF2/Merlin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NF2 Antibody (YA1386)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), DU145 (lane 2, 15 μg), HUVEC (lane 3, 15 μg), PANC-1 (lane 4, 15 μg), C6 (lane 5, 15 μg), and Mouse brain (lane 6, 15 μg) using NF2/Merlin Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NF2 Antibody (YA1386)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and Caco-2 (lane 6) using NF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NF2 Antibody (YA1386)
    Western blot analysis of extracts from HUVEC(lane 2(20μg), PANC-1(lane 3(20μg), A431(lane 4(20μg) and PC-3(lane 5(20μg) using NF2 Antibody (HY-P81641). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NF2 is a Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex

  • Subcellular Localization

    Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Nucleus; Cytoplasm, perinuclear region; Cytoplasmic granule; Cytoplasm, perinuclear region; Cytoplasmic granule; Nucleus; Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, perinuclear region; Cytoplasmic granule; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in human tissues, with the exception of human retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels

  • Isoforms & Post-Translational Modification

    P35240 has 10 isomers: P35240-1: 69690 Da (predicted); P35240-2: 72514 Da (predicted); P35240-3: 69090 Da (predicted); P35240-4: 59096 Da (predicted); P35240-5: 64189 Da (predicted); P35240-6: 63996 Da (predicted); P35240-7: 30454 Da (predicted); P35240-8: 65350 Da (predicted); P35240-9: 19215 Da (predicted); P35240-10: 24515 Da (predicted).
    Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741). The dephosphorylation of Ser-518 favors the interaction with NOP53 (PubMed:21167305);Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation

  • Subunit

    Interacts with NHERF1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1. Interacts (via FERM domain) with NOP53; the interaction is direct (PubMed:21167305). Interacts with SCHIP1; the interaction is direct (PubMed:10669747)

  • SwissProt ID

    P35240

  • Gene ID
    4771 [NCBI]
  • Synonyms

    ACN; BANF; Merlin; Moesin ezrin radixin like protein; Neurofibromatosis 2; Neurofibromin 2; Nf2; SCH; Schwannomerlin

  • Research Field

    Neuroscience

NF2 Antibody (YA1386) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), DU145 (lane 2, 15 μg), HUVEC (lane 3, 15 μg), PANC-1 (lane 4, 15 μg), C6 (lane 5, 15 μg), and Mouse brain (lane 6, 15 μg) using NF2/Merlin Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and Caco-2 (lane 6) using NF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC(lane 2(20μg), PANC-1(lane 3(20μg), A431(lane 4(20μg) and PC-3(lane 5(20μg) using NF2 Antibody (HY-P81641). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • NF2 Antibody (YA1386) Product Image
    1/16
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • NF2 Antibody (YA1386) Product Image
  • Experimental Validation Results for NF2 Antibody (YA1386)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), DU145 (lane 2, 15 μg), HUVEC (lane 3, 15 μg), PANC-1 (lane 4, 15 μg), C6 (lane 5, 15 μg), and Mouse brain (lane 6, 15 μg) using NF2/Merlin Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for NF2 Antibody (YA1386)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and Caco-2 (lane 6) using NF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NF2 Antibody (YA1386)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC(lane 2(20μg), PANC-1(lane 3(20μg), A431(lane 4(20μg) and PC-3(lane 5(20μg) using NF2 Antibody (HY-P81641). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • Experimental Validation Results for NF2 Antibody (YA1386)
  • NF2 Antibody (YA1386) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), DU145 (lane 2, 15 μg), HUVEC (lane 3, 15 μg), PANC-1 (lane 4, 15 μg), C6 (lane 5, 15 μg), and Mouse brain (lane 6, 15 μg) using NF2/Merlin Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • NF2 Antibody (YA1386) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and Caco-2 (lane 6) using NF2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NF2 Antibody (YA1386) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVEC(lane 2(20μg), PANC-1(lane 3(20μg), A431(lane 4(20μg) and PC-3(lane 5(20μg) using NF2 Antibody (HY-P81641). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • NF2 Antibody (YA1386) Product Image WB
  • NF2 Antibody (YA1386) Product Image WB
  • NF2 Antibody (YA1386) Product Image WB