Casein Kinase 1 alpha Antibody (YA564)
(Synonyms: CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1)Based on 1 Customer Validation
Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Casein Kinase 1 alpha Antibody (YA564) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 1 alpha.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 34 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 39 kDa
Entrez Gene: 1452 Human ; 93687 Mouse ; 113927 Rat
SwissProt: P48729 Human ; Q8BK63 Mouse ; P97633 Rat
OMIM: 600505 Human
Synthetic peptide corresponding to Human Casein Kinase 1 alpha.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using Casein Kinase 1 alpha (HY-P80585) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
Casein Kinase 1 alpha is a Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor. Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome, centromere, kinetochore; Nucleus speckle; Cytoplasm, cytoskeleton, cilium basal body; Cytoplasm, cytoskeleton, spindle
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Isoforms & Post-Translational Modification
P48729 has 3 isomers: P48729-1: 38915 Da (predicted); P48729-2: 41937 Da (predicted); P48729-3: 37567 Da (predicted).
Phosphorylated by MTOR in response to mitogenic stimulation, leading to its activation -
Subunit
Interacts with the Axin complex (PubMed:11955436). Interacts with TUT1, leading to TUT1 phosphorylation (PubMed:1409656).
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SwissProt ID
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Synonyms
CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1
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Research Field
Signal Transduction
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Liu C, et al. Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell. 2002 Mar 22;108(6):837-47. [Content Brief]
[2]. Brockman JL, et al. Cell cycle-dependent localization of casein kinase I to mitotic spindles. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9454-8. [Content Brief]
[3]. Gonzales ML, et al. CKIalpha is associated with and phosphorylates star-PAP and is also required for expression of select star-PAP target messenger RNAs. J Biol Chem. 2008 May 2;283(18):12665-73. [Content Brief]
[4]. Kuga T, et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer. J Cell Sci. 2013 Oct 15;126(Pt 20):4721-31. [Content Brief]
[5]. Gao D, et al. mTOR drives its own activation via SCF(βTrCP)-dependent degradation of the mTOR inhibitor DEPTOR. Mol Cell. 2011 Oct 21;44(2):290-303. [Content Brief]
[6]. Duan S, et al. mTOR generates an auto-amplification loop by triggering the βTrCP- and CK1α-dependent degradation of DEPTOR. Mol Cell. 2011 Oct 21;44(2):317-24. [Content Brief]
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