2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies
  5. Dopamine Transporter Antibody

Dopamine Transporter Antibody

Cat. No.: HY-P81165
COA
SDS
User Guide for Antibodies Technical Support

Dopamine Transporter Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Dopamine Transporter.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Descripciòn

Descripciòn

Dopamine Transporter Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Dopamine Transporter.
Host

Rabbit
Clonality

Polyclonal
Peso molecular
Predicted band size: 68 kDa;
Observed band size: 73 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat Predicted Reactivity: Dog, Cow
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human DAT1: 579-620/620
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-500
Sensitivity Endogenous Pureza affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from NIH3T3 (lane 1) and M-liver (lane 2) and M-brain (lane 3) using Dopamine Transporter antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Mediates sodium- and chloride-dependent transport of dopamine (PubMed:10375632, PubMed:11093780, PubMed:1406597, PubMed:15505207, PubMed:19478460, PubMed:39112701, PubMed:39112703, PubMed:39112705, PubMed:8302271). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity)
Subcellular Localization：Cell membrane; Multi-pass membrane protein; Cell projection, neuron projection; Cell projection, axon
Expression：
Tissue_specificity:Highly expressed in substantia nigra (PubMed:7637582) . Expressed in axonal varicosities in dopaminergic nerve terminals (at protein level) (PubMed:17296554) . Expressed in the striatum (at protein level) (PubMed:17296554)
Subunit：Monomer (PubMed:39112701, PubMed:39112703, PubMed:39112705). Homooligomer; disulfide-linked (Ref.16). Interacts with PRKCABP and TGFB1I1 (PubMed:11343649, PubMed:12177201). Interacts (via N-terminus) with SYNGR3 (via N-terminus) (By similarity). Interacts with SLC18A2 (By similarity). Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (PubMed:15505207). Interacts with alpha-synuclein/SNCA (PubMed:26442590). Interacts with SEPTIN4 (By similarity)
RRID
Database

Entrez Gene: 6531 Human ; 13162 Mouse ; 24898 Rat

SwissProt: Q01959 Human ; Q61327 Mouse ; P23977 Rat

OMIM: 613135 Human

Synonyms
DA transporter; DAT 1; DAT; DAT1; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); dopamine transporter; ADAT 1; Adenosine deaminase tRNA specific 1; HADAT1; tRNA specific adenosine deaminase 1.
Documentación
SDS (254 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Dopamine Transporter Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Dopamine Transporter Antibody
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HY-P81165
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