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Dopamine Transporter Antibody

(Synonyms: DA transporter; DAT 1; DAT; DAT1; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); dopamine transporter; ADAT 1; Adenosine deaminase tRNA specific 1; HADAT1; tRNA specific adenosine deaminase 1.)
Customer Review

Based on 1 Customer Validation

Dopamine Transporter Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Dopamine Transporter.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Dopamine Transporter Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1:100-500

Product Details

Description

Dopamine Transporter Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Dopamine Transporter.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Dog,Cow
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 73 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human DAT1: 579-620/620

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103251

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Dopamine Transporter Antibody
    Western blot analysis of extracts from NIH3T3 (lane 1) and M-liver (lane 2) and M-brain (lane 3) using Dopamine Transporter antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Dopamine Transporter Antibody
    Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    Immunocytochemistry analysis of PC-3 cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dopamine Transporter Antibody
    Immunocytochemistry analysis of U2OS cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Dopamine transporter (DAT) is a plasma membrane protein that mediates the reuptake of extracellular dopamine (DA) and controls the spatiotemporal dynamics of dopaminergic neurotransmission. DATs play a key role in terminating dopaminergic signalling and in maintaining a releasable pool of dopamine. DATs help to modulate the concentration of extraneuronal dopamine by actively shuttling released transmitter molecules back across the plasma membrane into dopaminergic neurons, where they can be sequestered for later reuse or enzymatic catabolism.

    DAT is a principle target of various psychostimulant, nootropic, and antidepressant drugs, as well as certain drugs used recreationally, including the notoriously addictive stimulant cocaine. DAT ligands have traditionally been divided into two categories: cocaine-like inhibitors and amphetamine-like substrates. DAT is regulated by multiple signaling systems, such as PKC.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cell projection, neuron projection; Cell projection, axon

  • Expression


    Tissue_specificity:Highly expressed in substantia nigra (PubMed:7637582) . Expressed in axonal varicosities in dopaminergic nerve terminals (at protein level) (PubMed:17296554) . Expressed in the striatum (at protein level) (PubMed:17296554)

  • Subunit

    Monomer (PubMed:39112701, PubMed:39112703, PubMed:39112705). Homooligomer; disulfide-linked (Ref.16). Interacts with PRKCABP and TGFB1I1 (PubMed:11343649, PubMed:12177201). Interacts (via N-terminus) with SYNGR3 (via N-terminus) (By similarity). Interacts with SLC18A2 (By similarity). Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (PubMed:15505207). Interacts with alpha-synuclein/SNCA (PubMed:26442590). Interacts with SEPTIN4 (By similarity)

  • SwissProt ID

    Q01959

  • Gene ID
    6531 [NCBI]
  • Synonyms

    DA transporter; DAT 1; DAT; DAT1; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); dopamine transporter; ADAT 1; Adenosine deaminase tRNA specific 1; HADAT1; tRNA specific adenosine deaminase 1.

Dopamine Transporter Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 1) and M-liver (lane 2) and M-brain (lane 3) using Dopamine Transporter antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dopamine Transporter Antibody Product Image
    1/16
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Dopamine Transporter Antibody Product Image
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH3T3 (lane 1) and M-liver (lane 2) and M-brain (lane 3) using Dopamine Transporter antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dopamine Transporter Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Experimental Validation Results for Dopamine Transporter Antibody
  • Dopamine Transporter Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH3T3 (lane 1) and M-liver (lane 2) and M-brain (lane 3) using Dopamine Transporter antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Dopamine Transporter Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Dopamine Transporter Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Dopamine Transporter Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat caudate tissue using Dopamine Transporter Antibody (HY-P81165, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Dopamine Transporter Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dopamine Transporter Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling Dopamine Transporter with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Dopamine Transporter Antibody (HY-P81165) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Dopamine Transporter Antibody Product Image WB
  • Dopamine Transporter Antibody Product Image IHC-P
  • Dopamine Transporter Antibody Product Image IHC-P
  • Dopamine Transporter Antibody Product Image IHC-P
  • Dopamine Transporter Antibody Product Image ICC
  • Dopamine Transporter Antibody Product Image ICC