NLK Antibody
(Synonyms: DKFZp761G1211, FLJ21033, LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK)NLK Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NLK.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-100 | 1:50-200 |
Product Details
NLK Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NLK.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 58 kDa;
Recombinant protein of human NLK (C-terminal ).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NLK is a Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner. Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Nucleus; Cytoplasm
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Isoforms & Post-Translational Modification
NLK has an amino acid length of 527, molecular weight is 58283 Da.在 Thr-298 位点发生磷酸化。Thr-298 位点的分子间自磷酸化可激活该酶NLK 的氨基酸个数为 527 个，分子量为 58283 Da。Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
DKFZp761G1211, FLJ21033, LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK
Documentation
[1]. Ishitani T, et al. The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling. Mol Cell Biol. 2003 Jan;23(1):131-9. [Content Brief]
[2]. Smit L, et al. Wnt activates the Tak1/Nemo-like kinase pathway. J Biol Chem. 2004 Apr 23;279(17):17232-40. [Content Brief]
[3]. Ohkawara B, et al. Role of the TAK1-NLK-STAT3 pathway in TGF-beta-mediated mesoderm induction. Genes Dev. 2004 Feb 15;18(4):381-6. [Content Brief]
[4]. Kojima H, et al. STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGFbeta-activated kinase 1 for STAT3 Ser-727 phosphorylation. Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4524-9. [Content Brief]
[5]. Kim S, et al. Regulation of FOXO1 by TAK1-Nemo-like kinase pathway. J Biol Chem. 2010 Mar 12;285(11):8122-9. [Content Brief]
[6]. Szypowska AA, et al. Oxidative stress-dependent regulation of Forkhead box O4 activity by nemo-like kinase. Antioxid Redox Signal. 2011 Feb 15;14(4):563-78. [Content Brief]
[7]. Togi S, et al. Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). J Biol Chem. 2011 May 27;286(21):19170-7. [Content Brief]
[8]. Ishitani T, et al. Nemo-like kinase suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. Nat Cell Biol. 2010 Mar;12(3):278-85. [Content Brief]
[9]. Kanei-Ishii C, et al. Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK. Genes Dev. 2004 Apr 1;18(7):816-29. [Content Brief]
[10]. Zhang ZY, et al. Stabilization of ATF5 by TAK1-Nemo-like kinase critically regulates the interleukin-1β-stimulated C/EBP signaling pathway. Mol Cell Biol. 2015 Mar;35(5):778-88. [Content Brief]
[11]. Yuan HX, et al. NLK phosphorylates Raptor to mediate stress-induced mTORC1 inhibition. Genes Dev. 2015 Nov 15;29(22):2362-76. [Content Brief]
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