WSTF Antibody (YA9735)
(Synonyms: baz1b, BAZ1B_HUMAN, Bromodomain adjacent to zinc finger domain protein 1B, hWALP 2, hWALP-2, hWALP2, transcription factor WSTF, Tyrosine-protein kinase BAZ1B, WALP-2, WALP2)WSTF Antibody (YA9735) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to WSTF.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:5000 | 1:100-250 |
Product Details
WSTF Antibody (YA9735) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to WSTF.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 171 kDa;
Recombinant protein of Human WSTF (aa 2-96).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
WSTF is an Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Regulatory subunit of the ATP-dependent WICH-1 and WICH-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template. The WICH-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the WICH-5 ISWI chromatin remodeling complex. The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription (By similarity). Within the B-WICH complex has a role in RNA polymerase III transcription. Mediates the recruitment of the WICH-5 ISWI chromatin remodeling complex to replication foci during DNA replication[1][2][3][4][5][6].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. -
Isoforms & Post-Translational Modification
WSTF has 2 isoforms, Q9UIG0-1: amino acid length is 1483, molecular weight is 170903 Da (predicted); Q9UIG0-2: amino acid length is 1479, molecular weight is 170447 Da (predicted).
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Subunit
Component of the WICH-1 ISWI chromatin remodeling complex, at least composed of SMARCA1 and BAZ1B/WSTF, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA.
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SwissProt ID
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Synonyms
baz1b, BAZ1B_HUMAN, Bromodomain adjacent to zinc finger domain protein 1B, hWALP 2, hWALP-2, hWALP2, transcription factor WSTF, Tyrosine-protein kinase BAZ1B, WALP-2, WALP2
Documentation
[1]. Xiao A, et al. WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature. 2009 Jan 1;457(7225):57-62. [Content Brief]
[2]. Cook PJ, et al. Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Nature. 2009 Apr 2;458(7238):591-6. [Content Brief]
[3]. Bozhenok L, et al. WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci. EMBO J. 2002 May 1;21(9):2231-41. [Content Brief]
[4]. Oppikofer M, et al. Expansion of the ISWI chromatin remodeler family with new active complexes. EMBO Rep. 2017 Oct;18(10):1697-1706. [Content Brief]
[5]. Cavellán E, et al. The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. J Biol Chem. 2006 Jun 16;281(24):16264-71. [Content Brief]
[6]. Poot RA, et al. The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nat Cell Biol. 2004 Dec;6(12):1236-44. [Content Brief]
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