MMP12 Antibody (YA288)
(Synonyms: Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME)
Based on 1 Customer Validation
MMP12 Antibody (YA288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP12.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Rat, Hamster
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
MMP12 Antibody (YA288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MMP12.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat, Hamster
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Observed Molecular WeightObserved band size: 54/45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 54 kDa
Synthetic peptide corresponding to Human MMP12.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from C6(lane 2(20ug) , HEK293T(lane 3(20ug) and MCF-7(lane 4(20ug) using MMP12 Antibody (HY-P80755) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
MMP-12, also known as macrophage metalloelastase, is a zinc-dependent extracellular matrix protease predominantly produced by activated macrophages and is characterized by potent elastolytic activity involved in tissue injury and remodeling processes[1][2]. Mechanistically, MMP-12 participates in extracellular matrix degradation, macrophage migration, inflammatory signaling, and tissue remodeling, thereby linking innate immune responses to structural alterations in affected tissues[2][3]. In disease settings, elevated MMP-12 activity has been associated with chronic pulmonary disorders, including chronic obstructive pulmonary disease and emphysema, where elastin degradation contributes directly to pathological lung remodeling[2][4]. Experimental studies further demonstrate that MMP-12 regulates macrophage infiltration, inflammatory cytokine expression, vascular dysfunction, and pathological angiogenesis in ischemic retinopathy models, supporting its broader role in inflammatory and remodeling-associated diseases[3]. Compared with several related matrix metalloproteinases that display broader substrate repertoires, MMP-12 is distinguished by its strong elastase activity and prominent expression in inflammatory macrophages, making it particularly relevant to elastin-rich tissue remodeling and macrophage-driven pathology[1][2]. For experimental applications, genetic deletion or pharmacological inhibition of MMP-12 reduces inflammatory responses, macrophage recruitment, and pathological tissue remodeling in multiple disease models, supporting its utility as a mechanistic target for studying inflammation, extracellular matrix turnover, and disease progression[3][4].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix
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Expression
Tissue_specificity:Found in alveolar macrophages but not in peripheral blood monocytes
Induction:By exposure to bacterial lipopolysaccharides (LPS) . Inhibited by dexamethasone -
SwissProt ID
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Synonyms
Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME
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Research Field
Cardiovascular
Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
[1]. Garbacki N, et al. Matrix metalloproteinase 12 silencing: a therapeutic approach to treat pathological lung tissue remodeling? Pulm Pharmacol Ther. 2009 Aug;22(4):267-78. [Content Brief]
[2]. Uniprotkb.
[3]. Li J, et al. Macrophage metalloelastase (MMP-12) deficiency mitigates retinal inflammation and pathological angiogenesis in ischemic retinopathy. PLoS One. 2012;7(12):e52699. [Content Brief]
[4]. Bhaskaran R, et al. Solution structure of inhibitor-free human metalloelastase (MMP-12) indicates an internal conformational adjustment. J Mol Biol. 2007 Dec 14;374(5):1333-44. [Content Brief]
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