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ACOX1 Antibody (YA1979)

(Synonyms: AOX; Acox; Paox; D130055E20Rik)
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Based on 1 Customer Validation

ACOX1 Antibody (YA1979) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACOX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACOX1 Antibody (YA1979)
  • ACOX1 Antibody (YA1979) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

ACOX1 Antibody (YA1979) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ACOX1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 74,48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Species Reactivity Database
Immunogen

Recombinant protein of mouse ACOX1 aa1-270.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104029

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACOX1 Antibody (YA1979)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using ACOX1 Antibody (HY-P82234) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ACOX1 is involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids. Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)); Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain lengtH2O2 10 carbons (decanoyl-CoA) in vitro; Is active against a much broader range of substrates and shows activity towards long-chain acyl-CoAs

  • Subcellular Localization

    Peroxisome

  • Expression


    Tissue_specificity:Highest levels of isoform 1 are found in liver and kidney while highest levels of isoform 2 are found in white adipose tissue. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 is expressed at higher levels in brain, heart, lung, muscle, white adipose tissue and testis

  • Isoforms & Post-Translational Modification

    Q9R0H0 has 2 isomers: Q9R0H0-1: 74649 Da (predicted); Q9R0H0-2: 74718 Da (predicted).

  • Subunit

    Homodimer (By similarity). Interacts with LONP2 (By similarity)

  • SwissProt ID

    Q9R0H0

  • Gene ID
    51 [NCBI]
  • Synonyms

    AOX; Acox; Paox; D130055E20Rik

  • Research Field

    Signal Transduction

ACOX1 Antibody (YA1979) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using ACOX1 Antibody (HY-P82234) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACOX1 Antibody (YA1979) Product Image
    1/16
  • Experimental Validation Results for ACOX1 Antibody (YA1979)
  • ACOX1 Antibody (YA1979) Product Image
  • Experimental Validation Results for ACOX1 Antibody (YA1979)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using ACOX1 Antibody (HY-P82234) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ACOX1 Antibody (YA1979)
  • ACOX1 Antibody (YA1979) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using ACOX1 Antibody (HY-P82234) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACOX1 Antibody (YA1979) Product Image WB