MRCK beta Antibody
(Synonyms: KIAA1124, CDC42BPB, Serine/threonine-protein kinase MRCK beta, CDC42-binding protein kinase beta, DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, CDC42BP-beta, MRCK beta, Myotonic dystrophy protein kinase-like beta)MRCK beta Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRCK beta.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
MRCK beta Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRCK beta.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 230 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 194 kDa
Synthetic peptide corresponding to the C-term region of human MRCK beta.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Livraison
Shipping with blue ice.
Background
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Function
MRCK beta is a Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity)[1][2][3].
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Subcellular Localization
Cytoplasm; Cell membrane; Cell junction; Cell projection, lamellipodium
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Expression
Tissue_Specificity: Expressed in all tissues examined, with high levels in heart, brain, placenta and lung. -
Isoforms & Post-Translational Modification
MRCK beta has an amino acid length of 1711, molecular weight is 194315 Da.
Proteolytically cleaved by caspases upon apoptosis induction. -
Subunit
Homodimer and homotetramer via the coiled coil regions.
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SwissProt ID
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Synonyms
KIAA1124, CDC42BPB, Serine/threonine-protein kinase MRCK beta, CDC42-binding protein kinase beta, DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, CDC42BP-beta, MRCK beta, Myotonic dystrophy protein kinase-like beta
Documentation
[1]. Tan I, et al. Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase. FEBS Lett. 2011 May 6;585(9):1260-8. [Content Brief]
[2]. Heikkila T, et al. Co-crystal structures of inhibitors with MRCKβ, a key regulator of tumor cell invasion. PLoS One. 2011;6(9):e24825. [Content Brief]
[3]. Tan I, et al. A tripartite complex containing MRCK modulates lamellar actomyosin retrograde flow. Cell. 2008 Oct 3;135(1):123-36. [Content Brief]
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