Reptin Antibody (YA9709)
(Synonyms: 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, 48-kDa TATA box-binding protein-interacting protein, 48-kDa TBP-interacting protein, 51 kDa erythrocyte cytosolic protein, CGI-46, EC=3.6.1.-, ECP-51, ECP51, Erythrocyte cytosolic protein, 51-KD)Reptin Antibody (YA9709) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to Reptin.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P
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Reactivity :
Human, Mouse, Rat, Monkey
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:2000 | 1:100 | 1:2000 |
Product Details
Reptin Antibody (YA9709) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to Reptin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat, Monkey
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Calculated Molecular Weight Predicted band size: 51 kDa;
Recombinant protein of Human RUVB2 (aa 130-321).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Reptin is a Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. May play a role in regulating the composition of the U5 snRNP complex[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Nucleus matrix; Nucleus, nucleoplasm; Cytoplasm; Membrane; Dynein axonemal particle
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Expression
Tissue_Specificity: Ubiquitously expressed. Highly expressed in testis and thymus. -
Isoforms & Post-Translational Modification
Reptin has 2 isoforms, Q9Y230-1: amino acid length is 463, molecular weight is 51157 Da (predicted); Q9Y230-2: amino acid length is 418, molecular weight is 46306 Da (predicted).
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Subunit
Forms homohexameric rings.
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SwissProt ID
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Synonyms
48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, 48-kDa TATA box-binding protein-interacting protein, 48-kDa TBP-interacting protein, 51 kDa erythrocyte cytosolic protein, CGI-46, EC=3.6.1.-, ECP-51, ECP51, Erythrocyte cytosolic protein, 51-KD
Documentation
References
[1]. Kanemaki M, et al. TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. J Biol Chem. 1999 Aug 6;274(32):22437-44. [Content Brief]
[2]. Puri T, et al. Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. J Mol Biol. 2007 Feb 9;366(1):179-92. [Content Brief]
[3]. López-Perrote A, et al. Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife. 2020 Nov 18;9:. [Content Brief]
[4]. Doyon Y, et al. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Mol Cell Biol. 2004 Mar;24(5):1884-96. [Content Brief]
[5]. Obri A, et al. ANP32E is a histone chaperone that removes H2A.Z from chromatin. Nature. 2014 Jan 30;505(7485):648-53. [Content Brief]
[6]. Jin J, et al. A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex. J Biol Chem. 2005 Dec 16;280(50):41207-12. [Content Brief]
[7]. Chen L, et al. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. J Biol Chem. 2011 Apr 1;286(13):11283-9. [Content Brief]
[8]. Wood MA, et al. An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol Cell. 2000 Feb;5(2):321-30. [Content Brief]
[9]. Weiske J, et al. The histidine triad protein Hint1 interacts with Pontin and Reptin and inhibits TCF-beta-catenin-mediated transcription. J Cell Sci. 2005 Jul 15;118(Pt 14):3117-29. [Content Brief]
[10]. Cho SG, et al. TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage. Mol Cell Biol. 2001 Dec;21(24):8398-413. [Content Brief]
[11]. Marza E, et al. Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription. EMBO Rep. 2015 Mar;16(3):332-40. [Content Brief]
[12]. Cloutier P, et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun. 2017 May 31;8:15615. [Content Brief]
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