Neuropilin-1 Antibody (YA3507)(PBS only)

Cat. No.: HY-P83810A: Data Sheet User Guide for Antibodies
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Neuropilin-1 Antibody (YA3507) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neuropilin-1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Neuropilin-1 Antibody (YA3507) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neuropilin-1.

Host

Mouse

Molecular Weight

Predicted band size: 103 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O14786

Gene ID

8829 [NCBI]

Immunogen

Synthesized peptide of human Neuropilin-1 (AA: 45-59).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:10688880, PubMed:9288753, PubMed:9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:10688880, PubMed:19805273, PubMed:9288753, PubMed:9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799); (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection; Binds VEGF-165 and may inhibit its binding to cells (PubMed:10748121, PubMed:26503042). May induce apoptosis by sequestering VEGF-165 (PubMed:10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity

Subcellular Localization：Secreted; Mitochondrion membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cytoplasm

Expression：
Tissue_specificity:Expression of isoforms 1 and 2 appears to have no overlap. Expressed in olfactory epithelium (protein level) (PubMed:33082293) . Expressed in fibroblasts (protein level) (PubMed:36213313) . Expressed in blood vessels of various tissues. In developing embryos, primarily present in the nervous system. In adult tissues, highly expressed in the heart and placenta; moderately expressed in the lungs, liver, skeletal muscle, kidneys, and pancreas; and lowly expressed in the adult brain (PubMed:10688880, PubMed:9529250) . Expressed in the central nervous system, including olfactory-related regions such as the olfactory tubercle and paraolfactory gyrus (PubMed:33082293) ; expression of isoforms 1 and 2 appears to have no overlap. Present in hepatocytes of the liver and distal and proximal convoluted tubules of the kidneys.

Isoforms & Post-Translational Modification：O14786 has 3 isomers: O14786-1: 103134 Da (predicted); O14786-2: 71935 Da (predicted); O14786-3: 68376 Da (predicted).

Subunit：Homodimer, and heterodimer with NRP2 (PubMed:17989695). Interacts with FER (By similarity). Interacts with PLXNB1 (PubMed:10520995). Interacts with VEGFA (PubMed:19805273, PubMed:26503042). Interacts with ABCB8/MITOSUR in mitochondria (PubMed:30623799)

Synonyms

NRP1; NP1; NRP; BDCA4; CD304; VEGF165R

Documentation

User Guide for Antibodies (1077 KB)