GNAQ Antibody (YA2938)(PBS only)

Cat. No.: HY-P83193A: Data Sheet User Guide for Antibodies
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GNAQ Antibody (YA2938) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNAQ.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

GNAQ Antibody (YA2938) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNAQ.

Host

Rabbit

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P50148

Gene ID

2776 [NCBI]

Immunogen

A synthetic peptide of human GNAQ

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed:27852822). Together with GNA11, required for heart development (By similarity)

Subcellular Localization：Cell membrane; Lipid-anchor; Golgi apparatus; Nucleus; Nucleus membrane

Expression：
Tissue_specificity:Predominantly expressed in ovary, prostate, testis and colon. Down-regulated in the peripheral blood lymphocytes (PBLs) of rheumatoid arthritis (at protein level)

Subunit：G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (PubMed:37991948). Interacts (GDP-bound form) with RIC8A (via C-terminus); promoting GNAQ folding and association with the plasma membrane (PubMed:32126208). Binds NHERF1 (PubMed:12193606). Forms a complex with PECAM1 and BDKRB2 (PubMed:18672896). Interacts with GAS2L2 (PubMed:23994616)

Research Field

Cardiovascular

Synonyms

GAQ; SWS; CMC1; G-ALPHA-q

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/lymphocyte-cell.html [Content Brief]