RFWD3 Antibody
(Synonyms: RNF201, RFWD3, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201)RFWD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RFWD3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
RFWD3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RFWD3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 85 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 85 kDa
Synthetic peptide corresponding to the center region of human RFWD3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RFWD3 is an E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA)[1][2][3][4][5][6][7][8].
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Subcellular Localization
Nucleus; Nucleus, PML body; Cytoplasm
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Isoforms & Post-Translational Modification
RFWD3 has an amino acid length of 774, molecular weight is 85094 Da.DNA 损伤时，ATM 或 ATR 会介导第 46 位丝氨酸(Ser-46)和第 63 位丝氨酸(Ser-63)的磷酸化。ATM 介导的磷酸化发生在 DNA 损伤的早期阶段，而 ATR 是后期阶段的主要激酶。ATM 和 ATR 介导的磷酸化是稳定 p53/TP53 所必需的。部分磷酸化过程依赖于 RPA2 的存在RFWD3 的氨基酸个数为 774 个，分子量为 85094 Da。Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Part of the phosphorylation depends upon RPA2 presence
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Subunit
Interacts with MDM2 and p53/TP53.
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SwissProt ID
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Synonyms
RNF201, RFWD3, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201
Documentation
[1]. Gong Z, et al. E3 ligase RFWD3 participates in replication checkpoint control. J Biol Chem. 2011 Jun 24;286(25):22308-13. [Content Brief]
[2]. Liu S, et al. RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. J Biol Chem. 2011 Jun 24;286(25):22314-22. [Content Brief]
[3]. Elia AE, et al. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks. Mol Cell. 2015 Oct 15;60(2):280-93. [Content Brief]
[4]. Feeney L, et al. RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health. Mol Cell. 2017 Jun 1;66(5):610-621.e4. [Content Brief]
[5]. Inano S, et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell. 2017 Jun 1;66(5):622-634.e8. [Content Brief]
[6]. Gallina I, et al. The ubiquitin ligase RFWD3 is required for translesion DNA synthesis. Mol Cell. 2021 Feb 4;81(3):442-458.e9. [Content Brief]
[7]. Knies K, et al. Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi anemia. J Clin Invest. 2017 Aug 1;127(8):3013-3027. [Content Brief]
[8]. Fu X, et al. RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 stability in response to DNA damage. Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4579-84. [Content Brief]
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