phospho-PAK4/5/6 (Ser474/Ser602/Ser560) Antibody (YA7330)

Cat. No.: HY-P87646: Data Sheet User Guide for Antibodies
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phospho-PAK4/5/6 (Ser474/Ser602/Ser560) Antibody (YA7330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-PAK4/5/6 (Ser474/Ser602/Ser560).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

phospho-PAK4/5/6 (Ser474/Ser602/Ser560) Antibody (YA7330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-PAK4/5/6 (Ser474/Ser602/Ser560).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 64,75,81 kDa;

Observed band size: 64,80,75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

O96013

Immunogen

A synthesized peptide derived from human PAK4 around the phosphorylation site of S474

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell adhesion turnover, cell migration, growth, proliferation or cell survival (PubMed:26598620). Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Promotes kinase-independent stabilization of RHOU, thereby contributing to focal adhesion disassembly during cell migration (PubMed:26598620)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Highest expression in prostate, testis and colon

Isoforms & Post-Translational Modification：O96013 has four isomers: O96013-1: 64072 Da (predicted); O96013-2: 47924 Da (predicted); O96013-3: 48268 Da (predicted); O96013-4: 54940 Da (predicted).
Autophosphorylated on serine residues when activated by CDC42/p21 (Ref丨Polyubiquitinated, leading to its proteasomal degradation

Subunit：Interacts with FGFR2 and GRB2 (By similarity)

Documentation

User Guide for Antibodies (1077 KB)