RPL3 Antibody
(Synonyms: OK/SW-cl.32, RPL3, Large ribosomal subunit protein uL3, 60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B)RPL3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPL3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
RPL3 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RPL3.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 46 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 46 kDa
Synthetic peptide corresponding to the center region of human RPL3.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RPL3 is a Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell[1][2][3][4].
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Subcellular Localization
Nucleus, nucleolus; Cytoplasm
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Isoforms & Post-Translational Modification
RPL3 has an amino acid length of 403, molecular weight is 46109 Da.第 245 位组氨酸(His-245)可被 METTL18 持续单甲基化(PubMed:23349634, PubMed:33693809, PubMed:35674491)。His-245 的甲基化通过减缓核糖体在酪氨酸密码子上的移动速度来调控翻译延伸：较慢的延伸速度为合成蛋白的正确折叠提供充足时间，防止富含酪氨酸的蛋白在细胞内聚集(PubMed:35674491)。该甲基化对于 RPL3 整合入核糖体并非必需(PubMed:33693809)RPL3 的氨基酸个数为 403 个，分子量为 46109 Da。Constitutively monomethylated at His-245 by METTL18 (PubMed:23349634, PubMed:33693809, PubMed:35674491). Methylation at His-245 regulates translation elongation by slowing ribosome traversal on tyrosine codons: slower elongation provides enough time for proper folding of synthesized proteins and prevents cellular aggregation of tyrosine-rich proteins (PubMed:35674491). It is not required for incorporation of RPL3 into ribosomes (PubMed:33693809)
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Subunit
Component of the large ribosomal subunit.
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SwissProt ID
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Synonyms
OK/SW-cl.32, RPL3, Large ribosomal subunit protein uL3, 60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B
Documentation
[1]. Odintsova TI, et al. Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing. J Protein Chem. 2003 Apr;22(3):249-58. [Content Brief]
[2]. Anger AM, et al. Structures of the human and Drosophila 80S ribosome. Nature. 2013 May 2;497(7447):80-5. [Content Brief]
[3]. Liang X, et al. Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. Nat Commun. 2020 Jul 15;11(1):3542. [Content Brief]
[4]. Matsuura-Suzuki E, et al. METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. Elife. 2022 Jun 8;11:. [Content Brief]
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