NOTCH4 Antibody (YA2557)

製品番号: HY-P82812: データシート User Guide for Antibodies
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NOTCH4 Antibody (YA2557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOTCH4.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	$222	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	$360	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

Synthetic products have potential research and development risk.

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

NOTCH4 Antibody (YA2557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOTCH4.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 210 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q99466

Gene ID

4855 [NCBI]

Immunogen

A synthetic peptide of human NOTCH4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Nucleus

Expression：
Tissue_specificity:This gene is highly expressed in the heart, moderately expressed in the lungs and placenta, and lowly expressed in the liver, skeletal muscle, kidneys, pancreas, spleen, lymph nodes, thymus, bone marrow, and fetal liver. It is not expressed in adult brain tissue or peripheral blood leukocytes.

Isoforms & Post-Translational Modification：Q99466 has 3 isomers: Q99466-1: 209622 Da (predicted); Q99466-2: 61262 Da (predicted); Q99466-3: 39617 Da (predicted).
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity);Phosphorylated

Subunit：Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4

Database

Entrez Gene: 4855 Human

SwissProt: Q99466 Human

OMIM: 164951 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

NOTCH4; INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/jurkat-cell.html [Content Brief]