CCL4 beta Antibody (YA2559)

製品番号: HY-P82814: データシート SDS COA User Guide for Antibodies
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CCL4 beta Antibody (YA2559) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCL4 beta.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

CCL4 beta Antibody (YA2559) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCL4 beta.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 10 kDa;

Observed band size: 10 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P13236

Gene ID

388372 [NCBI]

Immunogen

A synthesized peptide derived from human CCL4/MIP1 beta aa1-50/92.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of HeLa cells labeling CCL4 beta with CCL4 beta Antibody (HY-P82814) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CCL4 beta Antibody (HY-P82814) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling CCL4 beta with CCL4 beta Antibody (HY-P82814) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CCL4 beta Antibody (HY-P82814) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B

Subcellular Localization：Secreted

Expression：
Induction:By mitogens

Subunit：Homodimer and heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69)

RRID

AB_3104908

Database

Entrez Gene: 388372 Human ; 20303 Mouse ;

SwissProt: P13236 Human ; Q8NHW4 Human ; P14097 Mouse ;

OMIM: 182284 Human

Research Field

Immunology

Synonyms

ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)