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DHCR7 Antibody

(Synonyms: 7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; Sterol Delta; DHCR7_HUMAN.)
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Based on 1 Customer Validation

DHCR7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DHCR7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DHCR7 Antibody
  • DHCR7 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

DHCR7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to DHCR7.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Mouse Predicted Reactivity: Human,Rat,Chicken,Cow,Dog,Horse
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

Entrez Gene: 13360 Mouse

SwissProt: O88455 Mouse

Immunogen

KLH conjugated synthetic peptide derived from human DHCR7: 351-450/475

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105750

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DHCR7 Antibody
    Western blot analysis of extracts from Mouse Brain(lane 2(20μg), Mouse Liver(lane 3(20μg)and Mouse Kidney(lane 4(20μg) using DHCR7 Antibody (HY-P83647). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    DHCR7 is an Oxidoreductase that catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core. Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol. Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation; Component of the microsomal antiestrogen binding site (AEBS), a multiproteic complex at the ER membrane that consists of an association between cholestenol Delta-isomerase/EBP and DHCR7. This complex is responsible for cholesterol-5,6-epoxide hydrolase (ChEH) activity, which consists in the hydration of cholesterol-5,6-epoxides (5,6-EC) into cholestane-3beta,5alpha,6beta-triol (CT). The precise role of each component of this complex has not been described yet[1][2][3][4][5][6][7].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed. Most abundant in adrenal gland, liver, testis, and brain

  • Subunit

    Interacts with DHCR24; this interaction regulates DHCR7 activity (PubMed:25637936). Interacts with TMEM147 (PubMed:25637936)

  • SwissProt ID

    Q9UBM7

  • Gene ID
    1717 [NCBI]
  • Synonyms

    7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; Sterol Delta; DHCR7_HUMAN.

DHCR7 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse Brain(lane 2(20μg), Mouse Liver(lane 3(20μg)and Mouse Kidney(lane 4(20μg) using DHCR7 Antibody (HY-P83647). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DHCR7 Antibody Product Image
    1/16
  • Experimental Validation Results for DHCR7 Antibody
  • DHCR7 Antibody Product Image
  • Experimental Validation Results for DHCR7 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse Brain(lane 2(20μg), Mouse Liver(lane 3(20μg)and Mouse Kidney(lane 4(20μg) using DHCR7 Antibody (HY-P83647). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DHCR7 Antibody
  • DHCR7 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse Brain(lane 2(20μg), Mouse Liver(lane 3(20μg)and Mouse Kidney(lane 4(20μg) using DHCR7 Antibody (HY-P83647). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DHCR7 Antibody Product Image WB