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Cyclin D1/CCND1 Antibody (YA6255)

(Synonyms: CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene)
Customer Review

Based on 1 Customer Validation

Cyclin D1/CCND1 Antibody (YA6255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin D1/CCND1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Cyclin D1/CCND1 Antibody (YA6255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin D1/CCND1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719182

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Western blot analysis of extracts from Neuro-2a (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg) and SH-SY5Y (lane 5(20μg) using Cyclin D1/CCND1 Antibody (HY-P86563) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat cervix tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cyclin D1/CCND1 is a Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus; Cytoplasm; Nucleus membrane

  • Subunit

    Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex (PubMed:19237565, PubMed:8114739). The cyclin subunit imparts substrate specificity to the complex (PubMed:19237565, PubMed:20399237, PubMed:8302605, PubMed:9106657). Component of the ternary complex CCND1/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity (PubMed:9106657). Interacts directly with CDKN1B (By similarity). Can form similar complexes with either CDKN1A or CDKN2A (By similarity). Interacts with UHRF2; the interaction ubiquitinates CCND1 and appears to occur independently of phosphorylation (PubMed:21952639). Interacts with USP2 (PubMed:19917254). Interacts (via cyclin N-terminal domain) with INSM1 (via N-terminal region); the interaction competes with the binding of CCND1 to CDK4 during cell cycle progression and inhibits CDK4 activity (PubMed:16569215, PubMed:18417529, PubMed:19124461). Interacts with CDK4; the interaction is prevented with the binding of CCND1 to INSM1 during cell cycle progression (PubMed:19124461)

  • SwissProt ID

    P24385

  • Gene ID
    595 [NCBI]
  • Synonyms

    CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene

Cyclin D1/CCND1 Antibody (YA6255) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg) and SH-SY5Y (lane 5(20μg) using Cyclin D1/CCND1 Antibody (HY-P86563) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cervix tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    1/16
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Neuro-2a (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg) and SH-SY5Y (lane 5(20μg) using Cyclin D1/CCND1 Antibody (HY-P86563) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat cervix tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Experimental Validation Results for Cyclin D1/CCND1 Antibody (YA6255)
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Neuro-2a (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg) and SH-SY5Y (lane 5(20μg) using Cyclin D1/CCND1 Antibody (HY-P86563) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat cervix tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat endometrium tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Cyclin D1/CCND1 Antibody (HY-P86563, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Cyclin D1/CCND1 with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin D1/CCND1 Antibody (HY-P86563) at 1/600 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin D1/CCND1 Antibody (YA6255) Product Image WB
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image IHC-P
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image ICC
  • Cyclin D1/CCND1 Antibody (YA6255) Product Image ICC