Perforin Antibody (YA5638)
(Synonyms: Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; P1; PERF_HUMAN; perforin 1; pore forming protein; Perforin 1; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; Pore forming protein; prf1; SHGC-10760)Perforin Antibody (YA5638) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Perforin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, WB, ICC/IF, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:100-500 | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Perforin Antibody (YA5638) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Perforin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 61 kDa
Synthesized peptide derived from human Perforin AA range: 1-100
Protein G
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Perforin is a Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cytolytic granule; Secreted; Cell membrane; Multi-pass membrane protein; Endosome lumen
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Expression
Induction:Repressed by contact with target cells -
Subunit
Monomer, as soluble protein (PubMed:20889983, PubMed:21037563). Homooligomer; homooligomerizes to form a pore-forming ring (PubMed:20889983, PubMed:21037563)
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SwissProt ID
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Synonyms
Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; P1; PERF_HUMAN; perforin 1; pore forming protein; Perforin 1; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; Pore forming protein; prf1; SHGC-10760
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