Copy Link Print Information

SIRT6 Antibody (YA078)

(Synonyms: SIR2L6, SIRT6, NAD-dependent protein deacylase sirtuin-6, NAD-dependent protein deacetylase sirtuin-6, Protein mono-ADP-ribosyltransferase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6, hSIRT6)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

SIRT6 Antibody (YA078) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • SIRT6 Antibody (YA078) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) SIRT6 Antibody (YA078)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500 1:50-1:200 1:50-1:500 1:50-1:100

Product Details

Description

SIRT6 Antibody (YA078) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human SIRT6.AA range:306-355.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102427

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Western blot analysis of extracts from MCF-7(lane 2(20μg) , NIH/3T3(lane 3(20μg) ,C2C12(lane 4(20μg)and HepG2( lane 5(20μg) using SIRT6 Antibody (HY-P80322). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Flow cytometric analysis of 1X106 HeLa cells labeling SIRT6 Antibody (HY-P80322, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SIRT6 is a nuclear NAD+-dependent sirtuin that functions as a deacetylase, deacylase, and mono-ADP-ribosyltransferase, thereby maintaining chromatin integrity, DNA repair capacity, telomere stability, and metabolic homeostasis[1][2]. Mechanistically, SIRT6 regulates multiple signaling and transcriptional programs involved in aging, cellular stress responses, glucose and lipid metabolism, inflammation, and genome maintenance, placing it at the intersection of epigenetic regulation and cellular adaptation[2][5][3]. Through its effects on DNA damage repair, chromosome maintenance, and metabolic control, SIRT6 contributes to the preservation of cellular function and resistance to age-associated dysfunction[5][3]. In disease contexts, altered SIRT6 activity has been linked to cancer, cardiovascular disease, diabetes, and neurodegenerative disorders, while experimental studies demonstrate that SIRT6 deficiency induces mitochondrial dysfunction, oxidative stress, and broad transcriptional changes associated with brain aging and neurodegeneration[2][4]. Compared with related sirtuin isoforms, SIRT6 is distinguished by its predominant chromatin-associated functions and its combined deacetylase, deacylase, and mono-ADP-ribosyltransferase activities that directly couple NAD+ sensing to epigenetic and genome-stability pathways[1][5]. For experimental applications, increasing attention has focused on small-molecule SIRT6 modulators, including both activators and inhibitors, which provide useful tools for mechanistic studies and evaluation of SIRT6-dependent pathways in aging, metabolism, cancer, and neurodegenerative disease models[2][6].

  • Subcellular Localization

    Nucleus; Chromosome; Chromosome, telomere; Endoplasmic reticulum

  • Expression


    Induction:Down-regulated in a number of cancers, such as pancreatic cancer or colon carcinomas (PubMed:23217706) . Post-transcriptionally regulated by miR-766 (PubMed:23653361) . Expression is post-transcriptionally repressed by miR-122 (PubMed:26748705)

  • Subunit

    Homodimer; binds to nucleosomes and DNA ends as a homodimer (PubMed:31995034, PubMed:32538779). Interacts with RELA; interferes with RELA binding to target DNA (PubMed:19135889). Interacts with SMARCA5; promoting recruitment of SMARCA5/SNF2H to double-strand breaks (DSBs) sites (PubMed:23911928). Interacts with the mTORC2 complex; preventing the ability of SIRT6 to deacetylate FOXO1. Interacts with the CLOCK-BMAL1 complex; recruited by the CLOCK-BMAL1 complex to regulate expression of clock-controlled genes. Interacts with CSNK2A2; preventing CSNK2A2 localization to the nucleus (By similarity)

  • SwissProt ID

    Q8N6T7

  • Gene ID
    51548 [NCBI]
  • Synonyms

    SIR2L6, SIRT6, NAD-dependent protein deacylase sirtuin-6, NAD-dependent protein deacetylase sirtuin-6, Protein mono-ADP-ribosyltransferase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6, hSIRT6

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Uniprotkb.

[2]. Chen RR, et al. SIRT6 in health and diseases: From molecular mechanisms to therapeutic prospects. Pharmacol Res. 2025 Nov;221:107984. [Content Brief]

[3]. SIRT6 gene information from NCBI.

[4]. Smirnov D, et al. SIRT6 is a key regulator of mitochondrial function in the brain. Cell Death Dis. 2023 Jan 18;14(1):35. [Content Brief]

[5]. Li Y, et al. SIRT6 Widely Regulates Aging, Immunity, and Cancer. Front Oncol. 2022;12:861334.

View More

SIRT6 Antibody (YA078) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , NIH/3T3(lane 3(20μg) ,C2C12(lane 4(20μg)and HepG2( lane 5(20μg) using SIRT6 Antibody (HY-P80322). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SIRT6 Antibody (HY-P80322, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SIRT6 Antibody (YA078) Product Image
    1/16
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • SIRT6 Antibody (YA078) Product Image
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20μg) , NIH/3T3(lane 3(20μg) ,C2C12(lane 4(20μg)and HepG2( lane 5(20μg) using SIRT6 Antibody (HY-P80322). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SIRT6 Antibody (HY-P80322, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SIRT6 Antibody (YA078)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • Experimental Validation Results for SIRT6 Antibody (YA078)
  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20μg) , NIH/3T3(lane 3(20μg) ,C2C12(lane 4(20μg)and HepG2( lane 5(20μg) using SIRT6 Antibody (HY-P80322). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using SIRT6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80322, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling SIRT6 Antibody (HY-P80322, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SIRT6 Antibody (YA078) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling SIRT6 with SIRT6 Antibody (HY-P80322) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SIRT6 Antibody (HY-P80322) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SIRT6 Antibody (YA078) Product Image WB
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image IHC-P
  • SIRT6 Antibody (YA078) Product Image FC
  • SIRT6 Antibody (YA078) Product Image ICC
  • SIRT6 Antibody (YA078) Product Image ICC