Copy Link Print Information

PD-L2 Antibody (YA1584)

(Synonyms: PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273)
Customer Review

Based on 1 Customer Validation

PD-L2 Antibody (YA1584) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PD-L2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PD-L2 Antibody (YA1584)
  • PD-L2 Antibody (YA1584) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PD-L2 Antibody (YA1584) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PD-L2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 31 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PD-L2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103142

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PD-L2 Antibody (YA1584)
    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), U251 (lane 4, 20ug) and THP-1 (lane 5, 20ug) using PD-L2 Antibody (HY-P81839). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PD-L2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production

  • Subcellular Localization

    Secreted; Endomembrane system; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in the heart, placenta, pancreas, lungs, and liver, and lowly expressed in the spleen, lymph nodes, and thymus.

    Induction:Up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL4/interleukin-4

  • Isoforms & Post-Translational Modification

    Q9BQ51 has 3 isomers: Q9BQ51-1: 30957 Da (predicted); Q9BQ51-2: 20867 Da (predicted); Q9BQ51-3: 20745 Da (predicted).

  • Subunit

    Interacts with PDCD1

  • SwissProt ID

    Q9BQ51

  • Gene ID
    80380 [NCBI]
  • Synonyms

    PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273

  • Research Field

    Immunology

PD-L2 Antibody (YA1584) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), U251 (lane 4, 20ug) and THP-1 (lane 5, 20ug) using PD-L2 Antibody (HY-P81839). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PD-L2 Antibody (YA1584) Product Image
    1/16
  • Experimental Validation Results for PD-L2 Antibody (YA1584)
  • PD-L2 Antibody (YA1584) Product Image
  • Experimental Validation Results for PD-L2 Antibody (YA1584)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), U251 (lane 4, 20ug) and THP-1 (lane 5, 20ug) using PD-L2 Antibody (HY-P81839). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PD-L2 Antibody (YA1584)
  • PD-L2 Antibody (YA1584) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), U251 (lane 4, 20ug) and THP-1 (lane 5, 20ug) using PD-L2 Antibody (HY-P81839). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PD-L2 Antibody (YA1584) Product Image WB