Thyroid Hormone Receptor alpha Antibody (YA2573)

Cat. No.: HY-P82828: Data Sheet COA
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User Guide for Antibodies
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Thyroid Hormone Receptor alpha Antibody (YA2573) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thyroid Hormone Receptor alpha.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Thyroid Hormone Receptor alpha Antibody (YA2573) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Thyroid Hormone Receptor alpha Antibody (YA2573) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thyroid Hormone Receptor alpha.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P10827

Gene ID

7067 [NCBI]

Immunogen

A synthesized peptide derived from human THRA aa420-490/490.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Caco-2 (lane 1, 15 μg), SW480 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using Thyroid Hormone Receptor alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine; Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action

Subcellular Localization：Nucleus; Cytoplasm; Nucleus

Isoforms & Post-Translational Modification：P10827 has 4 isomers: P10827-1: 54816 Da (predicted); P10827-2: 46813 Da (predicted); P10827-3: 50747 Da (predicted); P10827-4: 50465 Da (predicted).

Subunit：Binds DNA as a dimer; homodimer and heterodimer with RXRB. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D. Interacts with AKAP13. Interacts with TP53INP2. Interacts with PER2. Isoform alpha-2 and isoform alpha-1 interact with TACC1, but the interaction with alpha-1 is weaker. The interaction with isoform alpha-1, but not alpha-2, is decreased in the presence of thyroid hormone T3 (PubMed:20078863)

RRID

AB_3104922

Database

Entrez Gene: 7067 Human

SwissProt: P10827 Human

OMIM: 614450 Human

Research Field

Signal Transduction

Synonyms

AR7; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; THRA; THRA1; THRA2

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Thyroid Hormone Receptor alpha Antibody (YA2573) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Thyroid Hormone Receptor alpha Antibody (YA2573)

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