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Thyroid Hormone Receptor alpha Antibody (YA2573)

(Synonyms: AR7; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; THRA; THRA1; THRA2)
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Based on 1 Customer Validation

Thyroid Hormone Receptor alpha Antibody (YA2573) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thyroid Hormone Receptor alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Thyroid Hormone Receptor alpha Antibody (YA2573)
  • Thyroid Hormone Receptor alpha Antibody (YA2573) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Thyroid Hormone Receptor alpha Antibody (YA2573) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thyroid Hormone Receptor alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database

Entrez Gene: 7067 Human

SwissProt: P10827 Human

Immunogen

A synthesized peptide derived from human THRA aa420-490/490.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104922

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Thyroid Hormone Receptor alpha Antibody (YA2573)
    Western blot analysis was performed on extracts from Caco-2 (lane 1, 15 μg), SW480 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using Thyroid Hormone Receptor alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Thyroid Hormone Receptor alpha is a Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine; Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action

  • Subcellular Localization

    Nucleus; Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    P10827 has 4 isomers: P10827-1: 54816 Da (predicted); P10827-2: 46813 Da (predicted); P10827-3: 50747 Da (predicted); P10827-4: 50465 Da (predicted).

  • Subunit

    Binds DNA as a dimer; homodimer and heterodimer with RXRB. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D. Interacts with AKAP13. Interacts with TP53INP2. Interacts with PER2. Isoform alpha-2 and isoform alpha-1 interact with TACC1, but the interaction with alpha-1 is weaker. The interaction with isoform alpha-1, but not alpha-2, is decreased in the presence of thyroid hormone T3 (PubMed:20078863)

  • SwissProt ID

    P10827

  • Gene ID
    7067 [NCBI]
  • Synonyms

    AR7; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; THRA; THRA1; THRA2

  • Research Field

    Signal Transduction

Thyroid Hormone Receptor alpha Antibody (YA2573) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Caco-2 (lane 1, 15 μg), SW480 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using Thyroid Hormone Receptor alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Thyroid Hormone Receptor alpha Antibody (YA2573) Product Image
    1/16
  • Experimental Validation Results for Thyroid Hormone Receptor alpha Antibody (YA2573)
  • Thyroid Hormone Receptor alpha Antibody (YA2573) Product Image
  • Experimental Validation Results for Thyroid Hormone Receptor alpha Antibody (YA2573)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Caco-2 (lane 1, 15 μg), SW480 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using Thyroid Hormone Receptor alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Thyroid Hormone Receptor alpha Antibody (YA2573)
  • Thyroid Hormone Receptor alpha Antibody (YA2573) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Caco-2 (lane 1, 15 μg), SW480 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using Thyroid Hormone Receptor alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Thyroid Hormone Receptor alpha Antibody (YA2573) Product Image WB