PSIP1/LEDGF Antibody (YA4948)

Cat. No.: HY-P85256: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PSIP1/LEDGF Antibody (YA4948) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PSIP1/LEDGF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PSIP1/LEDGF Antibody (YA4948) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PSIP1/LEDGF.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O75475

Gene ID

11168 [NCBI]

Immunogen

A synthesized peptide derived from human PSIP1. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Widely expressed. Highly expressed in the thymus. Expressed throughout the human brain. Expressed in neurons but not in astrocytes. Significantly elevated expression in the fetal brain compared to the human brain. In the human brain, expressed in the subventricular zone (SVZ) and hippocampus, undetectable in other areas. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate area.

Isoforms & Post-Translational Modification：O75475 has 3 isomers: O75475-1: 60103 Da (predicted); O75475-2: 37725 Da (predicted); O75475-3: 37268 Da (predicted).
Citrullinated by PADI4

Subunit：Monomer (PubMed:15895093). Interacts with IFRD1/PC4 (PubMed:9822615). Isoform 2 interacts with SFRS1 (PubMed:9885563). Isoform 1 interacts (via IBD domain) with POGZ (via IBM motif) and CDCA7L (via IBM motifs) (PubMed:19244240, PubMed:25082813, PubMed:29997176). Interacts (via IBD domain) with KMT2A (via IBM motifs) with a moderate affinity whereas interacts with the KMT2A-MEN1 complex with a greater affinity; MEN1 enhances interaction of KMT2A with PSIP1 (PubMed:22327296, PubMed:25082813, PubMed:25305204, PubMed:29997176). Interacts with fusion protein KMT2A-MLLT3 (PubMed:25305204). Interacts (via IBD domain) with IWS1 (via IBM motif), MED1 (via IBM motif) and DBF4 (via IBM motifs) (PubMed:29997176)

Synonyms

PSIP1; DFS70; LEDGF; PSIP2; PC4 and SFRS1-interacting protein; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; Transcriptional coactivator p75/p52

Documentation

User Guide for Antibodies (1077 KB)