Elongation Factor 1A1 Antibody (YA460)(PBS only)
(Synonyms: EEF1A1; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; GRAF-1EF; LENG7; PTI1; eEF1A-1)Elongation Factor 1A1 Antibody (YA460) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation Factor 1A1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
Elongation Factor 1A1 Antibody (YA460) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation Factor 1A1.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
Synthetic peptide corresponding to Human eEF1A1 aa434-447.
affinity purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from K562(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Elongation Factor 1A1 Antibody (HY-P80654) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
Elongation Factor 1A1 is a Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling; (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Nucleus; Nucleus, nucleolus; Cell membrane
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Expression
Induction:By homocysteine (HC) , may mediate accelerated synthesis of free thiol-containing proteins in response to HC-induced oxidative stress -
Subunit
Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA (PubMed:12426392, PubMed:12426393). Interacts with PARP1 (PubMed:17177976). Interacts with KARS1 (PubMed:18029264).
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SwissProt ID
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Synonyms
EEF1A1; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; GRAF-1EF; LENG7; PTI1; eEF1A-1
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Ferguson A, et al. Functional Dynamics within the Human Ribosome Regulate the Rate of Active Protein Synthesis. Mol Cell. 2015 Nov 5;60(3):475-86. [Content Brief]
[2]. Carelli JD, et al. Ternatin and improved synthetic variants kill cancer cells by targeting the elongation factor-1A ternary complex. Elife. 2015 Dec 10;4:. [Content Brief]
[3]. Wang HY, et al. Synthesis and single-molecule imaging reveal stereospecific enhancement of binding kinetics by the antitumour eEF1A antagonist SR-A3. Nat Chem. 2022 Dec;14(12):1443-1450. [Content Brief]
[4]. Juette MF, et al. Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes. Elife. 2022 Oct 20;11:. [Content Brief]
[5]. Oltion K, et al. An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes. Cell. 2023 Jan 19;186(2):346-362.e17. [Content Brief]
[6]. Maruyama T, et al. Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells. Clin Exp Immunol. 2007 Jan;147(1):164-75. [Content Brief]
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