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CD56 Antibody (YA3222)

(Synonyms: NCAM1; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56)
Customer Review

Based on 1 Customer Validation

CD56 Antibody (YA3222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD56.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • CD56 Antibody (YA3222) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50 1:50-1:100

Product Details

Description

CD56 Antibody (YA3222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD56.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 140,180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 95 kDa
Species Reactivity Database

Entrez Gene: 4684 Human

SwissProt: P13591 Human

Immunogen

A synthesized peptide derived from human NCAM aa801-858.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105587

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD56 Antibody (YA3222)
    Western blot analysis of extracts from HeLa(lane 2(20μg)) and Jurkat (lane 3(20μg)) using NCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD56 Antibody (YA3222)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD56 Antibody (HY-P83477, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD56 is a neural cell adhesion molecule (NCAM1) belonging to the immunoglobulin superfamily (IgSF). In the immune system, CD56 is a hallmark surface molecule of NK cells. CD56 is also expressed in some activated T cells (especially NKT-like cells) and participates in processes such as cell adhesion, immune synapse formation, and regulation of cytotoxic function.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cell membrane; Lipid-anchor, GPI-anchor; Cell membrane; Lipid-anchor, GPI-anchor; Secreted; Secreted

  • Isoforms & Post-Translational Modification

    CD56 has 6 isomers: P13591-2: 94574 Da (predicted); P13591-1: 93361 Da (predicted); P13591-3: 83770 Da (predicted); P13591-4: 80312 Da (predicted); P13591-5: 73473 Da (predicted); P13591-6: 40760 Da (predicted).
    Polysialylated at Asn-459 and Asn-488 by ST8SIA2 and ST8SIA4. Polysialylation modulates cell interactions by confering both attractive and repulsive properties that are highly regulated by ST8SIA2 and ST8SIA4. Polysialylation is formed on a-2,3-linked sialic acid of core glycans

  • Subunit

    (Microbial infection) Interacts with rabies virus glycoprotein

  • SwissProt ID

    P13591

  • Gene ID
    4684 [NCBI]
  • Synonyms

    NCAM1; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56

  • Research Field

    Neuroscience

CD56 Antibody (YA3222) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)) and Jurkat (lane 3(20μg)) using NCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD56 Antibody (HY-P83477, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD56 Antibody (YA3222) Product Image
    1/16
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • CD56 Antibody (YA3222) Product Image
  • Experimental Validation Results for CD56 Antibody (YA3222)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)) and Jurkat (lane 3(20μg)) using NCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD56 Antibody (YA3222)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD56 Antibody (HY-P83477, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • Experimental Validation Results for CD56 Antibody (YA3222)
  • CD56 Antibody (YA3222) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg)) and Jurkat (lane 3(20μg)) using NCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD56 Antibody (YA3222) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling CD56 Antibody (HY-P83477, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD56 Antibody (YA3222) Product Image WB
  • CD56 Antibody (YA3222) Product Image FC