Phospholamban Antibody (YA1622)

製品番号: HY-P81877: データシート SDS COA User Guide for Antibodies
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Phospholamban Antibody (YA1622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholamban.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Phospholamban Antibody (YA1622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholamban.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 6 kDa;

Observed band size: 12,24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P26678

Gene ID

5350 [NCBI]

Immunogen

Recombinant protein of human Phospholamban

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed:28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass membrane protein; Sarcoplasmic reticulum membrane; Single-pass membrane protein; Mitochondrion membrane; Single-pass membrane protein; Membrane; Single-pass membrane protein

Expression：
Tissue_specificity:Cardiac muscle (protein levels)

Subunit：Homopentamer (PubMed:16043693, PubMed:16897780). Can also form heterooligomers witH2O2her sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) regulators ARLN, ERLN, SLN and STRIT1/DWORF (PubMed:36523160).

RRID

AB_3103975

Database

Entrez Gene: 5350 Human ; 18821 Mouse ; 64672 Rat

SwissProt: P26678 Human ; P61014 Mouse ; P61016 Rat

OMIM: 609909 Human

Research Field

Signal Transduction

Synonyms

PLN; PLB; Cardiac phospholamban; PLB

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)