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Phospholamban Antibody (YA1622)

(Synonyms: PLN; PLB; Cardiac phospholamban; PLB)
Customer Review

Based on 1 Customer Validation

Phospholamban Antibody (YA1622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholamban.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Phospholamban Antibody (YA1622) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

Phospholamban Antibody (YA1622) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholamban.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 12,24 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 6 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human Phospholamban

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103975

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    Western blot analysis of extracts from Rat heart (lane 2, 20ug), Mouse heart (lane 3, 20ug), SH-SY5Y (lane 4, 20ug) and Hela (lane 5, 20ug) using Phospholamban Antibody (HY-P81877). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Phospholamban is a Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle. Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. Also inhibits the activity of ATP2A3/SERCA3. Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation. In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning[1].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass membrane protein; Sarcoplasmic reticulum membrane; Single-pass membrane protein; Mitochondrion membrane; Single-pass membrane protein; Membrane; Single-pass membrane protein

  • Expression


    Tissue_specificity:Cardiac muscle (protein levels)

  • Subunit

    Homopentamer (PubMed:16043693, PubMed:16897780). Can also form heterooligomers witH2O2her sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) regulators ARLN, ERLN, SLN and STRIT1/DWORF (PubMed:36523160).

  • SwissProt ID

    P26678

  • Gene ID
    5350 [NCBI]
  • Synonyms

    PLN; PLB; Cardiac phospholamban; PLB

  • Research Field

    Signal Transduction

References

[1]. Zhao YG, et al. The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. Mol Cell. 2017 Sep 21;67(6):974-989.e6. [Content Brief]

Phospholamban Antibody (YA1622) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart (lane 2, 20ug), Mouse heart (lane 3, 20ug), SH-SY5Y (lane 4, 20ug) and Hela (lane 5, 20ug) using Phospholamban Antibody (HY-P81877). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospholamban Antibody (YA1622) Product Image
    1/16
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Phospholamban Antibody (YA1622) Product Image
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat heart (lane 2, 20ug), Mouse heart (lane 3, 20ug), SH-SY5Y (lane 4, 20ug) and Hela (lane 5, 20ug) using Phospholamban Antibody (HY-P81877). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Experimental Validation Results for Phospholamban Antibody (YA1622)
  • Phospholamban Antibody (YA1622) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat heart (lane 2, 20ug), Mouse heart (lane 3, 20ug), SH-SY5Y (lane 4, 20ug) and Hela (lane 5, 20ug) using Phospholamban Antibody (HY-P81877). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospholamban Antibody (YA1622) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospholamban Antibody (YA1622) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Phospholamban Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospholamban Antibody (YA1622) Product Image WB
  • Phospholamban Antibody (YA1622) Product Image IHC-P
  • Phospholamban Antibody (YA1622) Product Image IHC-P