2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Modification Specific Antibodies
  5. DiMethyl-Histone H3 Antibody (YA783)

DiMethyl-Histone H3 Antibody (YA783)

Art. -Nr.: HY-P80643
User Guide for Antibodies Technical Support

DiMethyl-Histone H3 Antibody (YA783) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DiMethyl-Histone H3.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe Preis Verfügbarkeit
20 μL Anfrage über Preis & Lieferzeit
50 μL Anfrage über Preis & Lieferzeit
100 μL Anfrage über Preis & Lieferzeit

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Beschreibung

Beschreibung

DiMethyl-Histone H3 Antibody (YA783) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DiMethyl-Histone H3.
Host

Mouse
Clonality

Monoclonal
Molekulargewicht
Predicted band size: 15 kDa;
Observed band size: 15 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Histone H3 (Di Methyl Lys27).The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Reinheit affinity purified
Conjugation Non-conjugated Modification Methylated
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Background
Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
Subcellular Localization：Nucleus; Chromosome
Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)
Database

Entrez Gene: 8350 Human ; 319152 Mouse ;

SwissProt: P68431 Human ; P68433 Mouse ;

OMIM: 137800 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Dokumentation
User Guide for Antibodies (1077 KB)

DiMethyl-Histone H3 Antibody (YA783) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Ihre kürzlich angesehenen Produkte:

Online-Anfrage

Your information is safe with us. * Required Fields.

Produktname

  

Requested Quantity *

Name des Antragstellers *

 

Anrede

E-Mail-Addresse *

 

Telefonnummer *

Department

 

Name der Organisation *

City

Bundesland

Country or Region *

      

Bemerkungen

Massenanfrage

Inquiry Information

Produktname:
DiMethyl-Histone H3 Antibody (YA783)
Art. -Nr.:
HY-P80643
Menge:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Produktname

  

Lot #

Name des Antragstellers *

 

Country or Region *

E-Mail-Addresse *

     

Telefonnummer *

 

Name der Organisation *

Bemerkungen

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.