DiMethyl-Histone H3 Antibody (YA783)

Cat. No.: HY-P80643: FAQs
Data Sheet User Guide for Antibodies

DiMethyl-Histone H3 Antibody (YA783) is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to DiMethyl-Histone H3 (Lys27) (5F6). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock
10 μL	$70	Ask For Quote & Lead Time
50 μL	$185	Ask For Quote & Lead Time
100 μL	$300	Ask For Quote & Lead Time

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Biological Activity
Technical Parameters
Properties
Documentation

Description	DiMethyl-Histone H3 Antibody (YA783) is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to DiMethyl-Histone H3 (Lys27) (5F6). It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.
Background	DiMethyl-Histone H3 (Lys27) (5F6): Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Tag	Free
Gene ID	8350
Synonyms	H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Molecular Weight	Predicted band size: 15 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Research Field	Epigenetics and Nuclear Signaling
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Host	Mouse
Reactivity	Human, Mouse, Rat
SwissProt ID	P68431
Dilution Ratio	WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20
Application	WB, ICC/IF, IP
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG1
Immunogen	Synthetic peptide corresponding to Histone H3 (Di Methyl Lys27).The exact sequence is proprietary to MCE.
Documentation	Data Sheet (234 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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DiMethyl-Histone H3 Antibody (YA783)

Biological Activity

Technical Parameters

Properties

Documentation

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