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G-CSF Antibody (YA3307)

(Synonyms: G-CSF; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; MGI 2; Pluripoietin)
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Based on 1 Customer Validation

G-CSF Antibody (YA3307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to G-CSF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for G-CSF Antibody (YA3307)
  • G-CSF Antibody (YA3307) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

G-CSF Antibody (YA3307) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to G-CSF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human G-CSF aa150-207.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105670

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for G-CSF Antibody (YA3307)
    Western blot analysis of extracts from Hela (lane 2(20μg)) and K562 (lane 3(20μg)) using G-CSF Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Granulocyte colony stimulating factor (G-CSF; CSF 3) is a pro-inflammatory cytokine which primarily stimulates the survival, proliferation, differentiation and function of neutrophil granulocyte progenitor cells and mature neutrophils. Human G-CSF is secreted mainly by monocytes and macrophages, but is also produced by endothelial cells, fibroblasts, and bone marrow stromal cells. G-CSF binds and activates its cognate receptor G-CSFR on neutrophils and their progenitors, which initiates downstream signaling cascades that include the Jak-STAT and MAP kinase pathways. G-CSF is a major regulator of neutrophil development and contributes to the regulation of multipotent hematopoietic progenitors. In addition, G-CSF also influences the phenotype and function of mature neutrophils and does so by modulating expression of for example chemokine receptors, up-regulating phagocytosis and production of reactive oxygen species (ROS) and enhancing bactericidal activity of neutrophils. It is worth mentioning that G-CSF is an FDA- and EMA- approved drug commonly used to treat neutropenia and to mobilize bone marrow hematopoietic stem cells for transplantation.

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    P09919 has 3 isomers: P09919-1: 22293 Da (predicted); P09919-2: 21978 Da (predicted); P09919-3: 18434 Da (predicted).
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells)

  • Subunit

    Monomer

  • SwissProt ID

    P09919

  • Gene ID
    1440 [NCBI]
  • Synonyms

    G-CSF; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; MGI 2; Pluripoietin

  • Research Field

    Immunology

G-CSF Antibody (YA3307) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)) and K562 (lane 3(20μg)) using G-CSF Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • G-CSF Antibody (YA3307) Product Image
    1/16
  • Experimental Validation Results for G-CSF Antibody (YA3307)
  • G-CSF Antibody (YA3307) Product Image
  • Experimental Validation Results for G-CSF Antibody (YA3307)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)) and K562 (lane 3(20μg)) using G-CSF Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for G-CSF Antibody (YA3307)
  • G-CSF Antibody (YA3307) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg)) and K562 (lane 3(20μg)) using G-CSF Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • G-CSF Antibody (YA3307) Product Image WB