PKA RI alpha Antibody (YA1751)

Cat. No.: HY-P82006: Data Sheet COA
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PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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PKA RI alpha Antibody (YA1751) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P10644

Gene ID

5573 [NCBI]

Immunogen

A synthetic peptide of human Protein Kinase A regulatory subunit I alpha

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using PKA RI alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from U87 (lane 2(20μg) and U87 (lane 3(40μg) using PKA RI alpha (HY-P82006) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells

Subcellular Localization：Cell membrane

Expression：
Tissue_specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible

Isoforms & Post-Translational Modification：P10644 has 2 isomers: P10644-1: 42982 Da (predicted); P10644-2: 38003 Da (predicted).
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity

Subunit：The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKACA and PRKACB (PubMed:33058759).

RRID

AB_3104127

Database

Entrez Gene: 5573 Human ;

SwissProt: P10644 Human ; Q5REL1Orangutan ;

OMIM: 160980 Human

Research Field

Signal Transduction

Synonyms

PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1

각종 서류

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PKA RI alpha Antibody (YA1751) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PKA RI alpha Antibody (YA1751)

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PKA RI alpha Antibody (YA1751) Related Classifications

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