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PKA RI alpha Antibody (YA1751)

(Synonyms: PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1)
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Based on 1 Customer Validation

PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • PKA RI alpha Antibody (YA1751) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Protein Kinase A regulatory subunit I alpha

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104127

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using PKA RI alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
    Western blot analysis of extracts from U87 (lane 2(20μg) and U87 (lane 3(40μg) using PKA RI alpha (HY-P82006) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PKA RI alpha is a Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible

  • Isoforms & Post-Translational Modification

    P10644 has 2 isomers: P10644-1: 42982 Da (predicted); P10644-2: 38003 Da (predicted).
    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity

  • Subunit

    The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKACA and PRKACB (PubMed:33058759).

  • SwissProt ID

    P10644

  • Gene ID
    5573 [NCBI]
  • Synonyms

    PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1

  • Research Field

    Signal Transduction

PKA RI alpha Antibody (YA1751) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using PKA RI alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg) and U87 (lane 3(40μg) using PKA RI alpha (HY-P82006) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PKA RI alpha Antibody (YA1751) Product Image
    1/16
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • PKA RI alpha Antibody (YA1751) Product Image
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using PKA RI alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from U87 (lane 2(20μg) and U87 (lane 3(40μg) using PKA RI alpha (HY-P82006) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • Experimental Validation Results for PKA RI alpha Antibody (YA1751)
  • PKA RI alpha Antibody (YA1751) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Rat brain (lane 2, 15 μg), C6 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and U-87MG (lane 5, 15 μg) using PKA RI alpha Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PKA RI alpha Antibody (YA1751) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from U87 (lane 2(20μg) and U87 (lane 3(40μg) using PKA RI alpha (HY-P82006) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PKA RI alpha Antibody (YA1751) Product Image WB
  • PKA RI alpha Antibody (YA1751) Product Image WB