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Kallikrein 2 Antibody (YA2729)

(Synonyms: hK2; hGK-1; KLK2A2)
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Based on 1 Customer Validation

Kallikrein 2 Antibody (YA2729) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kallikrein 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Kallikrein 2 Antibody (YA2729)
  • Kallikrein 2 Antibody (YA2729) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:50-1:200

Product Details

Description

Kallikrein 2 Antibody (YA2729) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kallikrein 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KLK2 aa210-261/261.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105102

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kallikrein 2 Antibody (YA2729)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) and MCF-7(lane 3(40ug) using Kallikrein 2 Antibody (HY-P82984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Kallikrein 2 is a Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin

  • Isoforms & Post-Translational Modification

    P20151 has 4 isomers: P20151-1: 28671 Da (predicted); P20151-2: 24674 Da (predicted); P20151-3: 18162 Da (predicted); P20151-4: 17301 Da (predicted).

  • SwissProt ID

    P20151

  • Gene ID
    3817 [NCBI]
  • Synonyms

    hK2; hGK-1; KLK2A2

  • Research Field

    Signal Transduction

Kallikrein 2 Antibody (YA2729) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) and MCF-7(lane 3(40ug) using Kallikrein 2 Antibody (HY-P82984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Kallikrein 2 Antibody (YA2729) Product Image
    1/16
  • Experimental Validation Results for Kallikrein 2 Antibody (YA2729)
  • Kallikrein 2 Antibody (YA2729) Product Image
  • Experimental Validation Results for Kallikrein 2 Antibody (YA2729)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) and MCF-7(lane 3(40ug) using Kallikrein 2 Antibody (HY-P82984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Kallikrein 2 Antibody (YA2729)
  • Kallikrein 2 Antibody (YA2729) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) and MCF-7(lane 3(40ug) using Kallikrein 2 Antibody (HY-P82984) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Kallikrein 2 Antibody (YA2729) Product Image WB