Pirh2 Antibody (YA1743)

Cat. No.: HY-P81998: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Pirh2 Antibody (YA1743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pirh2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Pirh2 Antibody (YA1743) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Pirh2 Antibody (YA1743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pirh2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 30 kDa;

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96PM5

Gene ID

25898 [NCBI]

Immunogen

A synthetic peptide of human Pirh2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg), Raji (lane 5(20μg) using Integrin alpha 9 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed:16914734, PubMed:17721809, PubMed:18006823, PubMed:19043414, PubMed:19483087, PubMed:21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed:19043414, PubMed:19483087). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed:21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes: RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed:33909987); Has no E3 ubiquitin-protein ligase activity

Subcellular Localization：Nucleus; Nucleus speckle; Cytoplasm

Expression：
Induction:Up-regulated during the S phase of the cell cycle (PubMed:18006823) . Expressed at low levels during G phase (PubMed:18006823) ; Down-regulated in hepatocellular carcinoma

Isoforms & Post-Translational Modification：Q96PM5 has 8 isomers: Q96PM5-1: 30110 Da (predicted); Q96PM5-2: 28983 Da (predicted); Q96PM5-3: 20720 Da (predicted); Q96PM5-4: 21697 Da (predicted); Q96PM5-5: 8494 Da (predicted); Q96PM5-6: 27676 Da (predicted); Q96PM5-7: 25337 Da (predicted); Q96PM5-8: 24210 Da (predicted).
Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability

Subunit：Monomer and homodimer. Interacts with AR, MDM2, KAT5, PLAG1, PLAGL2, COPE, UBE2D2 and GORAB/NTKLBP1

Database

Entrez Gene: 25898 Human

SwissProt: Q96PM5 Human

OMIM: 607680 Human

Research Field

Cell Biology

Synonyms

RCHY1; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; RING finger and CHY zinc finger domain-containing protein 1; Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Pirh2 Antibody (YA1743) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pirh2 Antibody (YA1743)

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