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  5. Pirh2 Antibody (YA1743)

Pirh2 Antibody (YA1743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pirh2.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

Pirh2 Antibody (YA1743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pirh2.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 30 kDa;
Observed band size: 30 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Pirh2
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg), Raji (lane 5(20μg) using Integrin alpha 9 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background
Function：E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed:16914734, PubMed:17721809, PubMed:18006823, PubMed:19043414, PubMed:19483087, PubMed:21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed:19043414, PubMed:19483087). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed:21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes: RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed:33909987); Has no E3 ubiquitin-protein ligase activity
Subcellular Localization：Nucleus; Nucleus speckle; Cytoplasm
Expression：
Induction:Up-regulated during the S phase of the cell cycle (PubMed:18006823) . Expressed at low levels during G phase (PubMed:18006823) ; Down-regulated in hepatocellular carcinoma
Isoforms & Post-Translational Modification：Q96PM5 has 8 isomers: Q96PM5-1: 30110 Da (predicted); Q96PM5-2: 28983 Da (predicted); Q96PM5-3: 20720 Da (predicted); Q96PM5-4: 21697 Da (predicted); Q96PM5-5: 8494 Da (predicted); Q96PM5-6: 27676 Da (predicted); Q96PM5-7: 25337 Da (predicted); Q96PM5-8: 24210 Da (predicted).
Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability
Subunit：Monomer and homodimer. Interacts with AR, MDM2, KAT5, PLAG1, PLAGL2, COPE, UBE2D2 and GORAB/NTKLBP1
Database

Entrez Gene: 25898 Human

SwissProt: Q96PM5 Human

OMIM: 607680 Human

Research Field

Cell Biology
Synonyms
RCHY1; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; RING finger and CHY zinc finger domain-containing protein 1; Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger
각종 서류
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Pirh2 Antibody (YA1743) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Pirh2 Antibody (YA1743)
Cat. No.:
HY-P81998
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SAFETY DATA SHEET (SDS)

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