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Phospho-STAT3 (Ser727) Antibody (YA147)

(Synonyms: APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

Phospho-STAT3 (Ser727) Antibody (YA147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT3 (Ser727).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human, Rat, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

Phospho-STAT3 (Ser727) Antibody (YA147) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT3 (Ser727).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat, Mouse
  • Observed Molecular Weight
    Observed band size: 88 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser727 of Human STAT3.AA range:701-750.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102402

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    STAT3 is a widely expressed STAT family transcription factor that regulates cellular growth, differentiation, survival, immunity, hematopoiesis, and cell migration[1]. Mechanistically, cytokines and growth factors activate JAKs, phosphorylate STAT proteins, promote STAT dimerization, and drive nuclear transcriptional regulation[2]. In inflammatory disease models, STAT3 shows strong tyrosine phosphorylation in human ulcerative colitis, Crohn’s disease, and DSS-induced mouse colitis, where IL-6 deficiency reduces colitis and STAT3 activation[3]. In cancer models, persistent STAT3 activation supports proliferation, survival, invasion, metastasis, immune evasion, and drug resistance, making STAT3 signaling a practical axis for tumor biology research[1][4]. Compared with related isoforms, STAT1 knockout mice mainly show impaired interferon signaling, while STAT3 is emphasized in IL-6-linked inflammation, tumor progression, and Th17-related immune dysfunction[2][5]. For experimental applications, JAK/STAT inhibitors such as AG490 and AZD1480 reduce STAT1/STAT3 activation in renal ischemia-reperfusion and α-synuclein neuroinflammation models[6][7].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Heart, brain, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. Expressed in naive CD4 (+) T cells as well as helper T cells Th17, Th1, and Th2 (PubMed:31899195) .

  • Subunit

    Forms a homodimer or a heterodimer with a related family member (at least STAT1) (PubMed:15653507, PubMed:28065600). Component of a promoter-binding complex composed of STAT3, NFATC3 and NFATC4; complex formation is enhanced by calcineurin (By similarity). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1 (By similarity) (PubMed:15194700, PubMed:15467733, PubMed:15677474, PubMed:15994929, PubMed:17344214). Interacts with IL23R in presence of IL23 (PubMed:12023369). Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression (By similarity). Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import (By similarity). Interacts with CAV2; the interaction is increased on insulin-induced tyrosine phosphorylation of CAV2 and leads to STAT3 activation (By similarity). Interacts with ARL2BP; interaction is enhanced with ARL2 (PubMed:18234692). Interacts with NEK6 (By similarity). Binds to CDK9 when activated and nuclear (PubMed:17956865). Interacts with BMX (PubMed:10688651). Interacts with ZIPK/DAPK3 (PubMed:16219639). Interacts with PIAS3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3 (PubMed:9388184). In prostate cancer cells, interacts with PRKCE and promotes DNA binding activity of STAT3 (PubMed:17875724). Interacts with STMN3, antagonizing its microtubule-destabilizing activity (By similarity). Interacts with the 'Lys-129' acetylated form of BIRC5/survivin (PubMed:20826784). Interacts with FER (PubMed:19147545). Interacts (via SH2 domain) with EIF2AK2/PKR (via the kinase catalytic domain) (PubMed:23084476). Interacts with INPP5F; the interaction is independent of STAT3 Tyr-705 phosphorylation status (PubMed:25476455). Interacts with FGFR4 (PubMed:26675719). Interacts with OCIAD1 (By similarity). Interacts with OCIAD2 (PubMed:29743632). Interacts (unphosphorylated or phosphorylated at Ser-727) with PHB1 (PubMed:31899195). Interacts and may form heterodimers with NHLH1 (By similarity). Found in a complex with SLC39A6, SLC39A10 and with the 'Ser-727' phosphorylated form of STAT3 throughout mitosis (PubMed:32797246). Interacts (when phosphorylated at Tyr-705) with CD274/PD-L1; promoting nuclear translocation of CD274/PD-L1 (PubMed:32929201). Interacts (when acetylated) with EP300 (via bromo domain); interaction takes place following STAT3 acetylation by EP300 and promotes enhanceosome assembly (PubMed:18782771). Interacts (when acetylated) with BRD2 (via bromo domain); interaction promotes STAT3 recruitment to chromatin and T-helper Th17 cell differentiation (PubMed:28262505). Interacts with FAM220A/SIPAR; the interaction occurs in both the nucleus and the cytoplasm, is enhanced by IL6 and promotes STAT3 dephosphorylation (By similarity). Interacts in both unphosphorylated and phosphorylated forms with FAM220A but interacts preferentially in the phosphorylated form in the nucleus (By similarity). Interacts with PTPN2; the interaction is promoted by FAM220A and leads to STAT3 dephosphorylation which negatively regulates STAT3 transcriptional activator activity (By similarity)

  • SwissProt ID

    P40763

  • Gene ID
    6774 [NCBI]
  • Synonyms

    APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

  • Research Field

    Signal Transduction

[1]. Liu Y, et al. STAT3 and its targeting inhibitors in osteosarcoma. Cell Prolif. 2021 Feb;54(2):e12974. [Content Brief]

[2]. Heim MH. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120. doi: 10.3109/10799899909036638. PMID: 10071751. et al. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):75-120. [Content Brief]

[3]. Suzuki A, et al. CIS3/SOCS3/SSI3 plays a negative regulatory role in STAT3 activation and intestinal inflammation. J Exp Med. 2001 Feb 19;193(4):471-81. [Content Brief]

[4]. Wu CJ, et al. Activation of STAT3 and STAT5 Signaling in Epithelial Ovarian Cancer Progression: Mechanism and Therapeutic Opportunity. Cancers (Basel). 2019 Dec 19;12(1):24. [Content Brief]

[5]. Tolomeo M, et al. The STAT Signaling Pathway in HIV-1 Infection: Roles and Dysregulation. Int J Mol Sci. 2025 Sep 18;26(18):9123. [Content Brief]

[6]. Si Y, et al. Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. J Transl Med. 2013 Jun 9;11:141. [Content Brief]

[7]. Qin H, et al. Inhibition of the JAK/STAT Pathway Protects Against α-Synuclein-Induced Neuroinflammation and Dopaminergic Neurodegeneration. J Neurosci. 2016 May 4;36(18):5144-59. [Content Brief]

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Phospho-STAT3 (Ser727) Antibody (YA147) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    1/16
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Experimental Validation Results for Phospho-STAT3 (Ser727) Antibody (YA147)
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , NIH/3T3 (lane 3(20μg) , Jurkat (lane 4(20μg),using Phospho-STAT3 (Ser727) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Heal cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-STAT3 (Ser727) with Phospho-STAT3 (Ser727) Antibody (HY-P80281) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-STAT3 (Ser727)Antibody (HY-P80281)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Phospho-STAT3 (Ser727) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80281, 1:500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image WB
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image ICC
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image ICC
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image IHC-P
  • Phospho-STAT3 (Ser727) Antibody (YA147) Product Image IHC-P