Copy Link Print Information

JAK2 Antibody (YA330)

(Synonyms: JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2)
4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

JAK2 Antibody (YA330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JAK2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • JAK2 Antibody (YA330) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) JAK2 Antibody (YA330)

More

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

JAK2 Antibody (YA330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JAK2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 131 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 131 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human JAK2.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3669006

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for JAK2 Antibody (YA330)
    Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80727, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    Western blot analysis of extracts from K562 (lane 2(20μg) and K562(lane 3(40μg) using JAK2 (HY-P80727) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    Western blot analysis of extracts from K562 (lane 2(20μg), 3T3 (lane 3(20μg), THP-1 (lane 4(20μg),using JAK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody (HY-P80727) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:15690087, PubMed:7615558, PubMed:9657743, PubMed:15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:15690087, PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed:9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed:25644777)

  • Subcellular Localization

    Endomembrane system; Peripheral membrane protein; Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Commonly expressed in most organizations

  • Subunit

    Interacts with EPOR, LYN, SIRPA, SH2B1 and TEC (By similarity). Interacts with IL23R (PubMed:12023369). Interacts with SKB1 (PubMed:10531356). Interacts with STAM2 (PubMed:10899310). Interacts with IFNGR2 (via intracellular domain) (PubMed:7615558, PubMed:7673114). Interacts with LEPR (Isoform B) (By similarity). Interacts with HSP90AB1; promotes functional activation in a heat shock-dependent manner (PubMed:20353823). Interacts with STRA6 (PubMed:21368206). Interacts with RHEX; this interaction occurs in a erythropoietin (EPO)-dependent manner (PubMed:25092874). Interacts with ASB2; the interaction targets JAK2 for Notch-induced proteasomal degradation (PubMed:21119685). Interacts with MPL/TPOR (PubMed:15899890)

  • SwissProt ID

    O60674

  • Gene ID
    3717 [NCBI]
  • Synonyms

    JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2

  • Research Field

    Cell Biology

JAK2 Antibody (YA330) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80727, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562(lane 3(40μg) using JAK2 (HY-P80727) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), 3T3 (lane 3(20μg), THP-1 (lane 4(20μg),using JAK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody (HY-P80727) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • JAK2 Antibody (YA330) Product Image
    1/16
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • JAK2 Antibody (YA330) Product Image
  • Experimental Validation Results for JAK2 Antibody (YA330)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80727, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg) and K562(lane 3(40μg) using JAK2 (HY-P80727) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), 3T3 (lane 3(20μg), THP-1 (lane 4(20μg),using JAK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody (HY-P80727) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • Experimental Validation Results for JAK2 Antibody (YA330)
  • JAK2 Antibody (YA330) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80727, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • JAK2 Antibody (YA330) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg) and K562(lane 3(40μg) using JAK2 (HY-P80727) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • JAK2 Antibody (YA330) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), 3T3 (lane 3(20μg), THP-1 (lane 4(20μg),using JAK2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • JAK2 Antibody (YA330) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 1(20μg)) 、THP-1 (lane 2(20μg)) 、Jurkat (lane 3(20μg)) and NIH-3T3 (lane 4(20μg)) using Jak2 Antibody (HY-P80727) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • JAK2 Antibody (YA330) Product Image WB
  • JAK2 Antibody (YA330) Product Image WB
  • JAK2 Antibody (YA330) Product Image WB
  • JAK2 Antibody (YA330) Product Image WB