MUS81 Antibody (YA9006)
(Synonyms: Crossover junction endonuclease MUS81, MUS81)
Based on 1 Customer Validation
MUS81 Antibody (YA9006) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MUS81.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human, mouse, rat
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
MUS81 Antibody (YA9006) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MUS81.
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Host Mouse
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Species Reactivityhuman, mouse, rat
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Observed Molecular WeightObserved band size: 72 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 62 kDa
OMIM: 606591
A synthesized peptide derived from human MUS81.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
MUS81 is a Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability. Both endonucleases have essentially the same substrate specificity though MUS81-EME2 is more active than its MUS81-EME1 counterpart. Both cleave 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs. MUS81-EME2 which is active during the replication of DNA is more specifically involved in replication fork processing. Replication forks frequently encounter obstacles to their passage, including DNA base lesions, DNA interstrand cross-links, difficult-to-replicate sequences, transcription bubbles, or tightly bound proteins. One mechanism for the restart of a stalled replication fork involves nucleolytic cleavage mediated by the MUS81-EME2 endonuclease. By acting upon the stalled fork, MUS81-EME2 generates a DNA double-strand break (DSB) that can be repaired by homologous recombination, leading to the restoration of an active fork. MUS81-EME2 could also function in telomere maintenance. MUS81-EME1, on the other hand, is active later in the cell cycle and functions in the resolution of mitotic recombination intermediates including the Holliday junctions, the four-way DNA intermediates that form during homologous recombination[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Nucleus, nucleolus
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Expression
Tissue_Specificity: Widely expressed.
Induction: Up-regulated in cells treated with agents that damage DNA or block replication. This up-regulation seems to be independent of transcription. -
Isoforms & Post-Translational Modification
MUS81 has an amino acid length of 551, molecular weight is 61173 Da.
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Subunit
Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME1.
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SwissProt ID
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Synonyms
Crossover junction endonuclease MUS81, MUS81
Documentation
References
[1]. Chen XB, et al. Human Mus81-associated endonuclease cleaves Holliday junctions in vitro. Mol Cell. 2001 Nov;8(5):1117-27. [Content Brief]
[2]. Constantinou A, et al. Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities. EMBO J. 2002 Oct 15;21(20):5577-85. [Content Brief]
[3]. Oğrünç M, et al. Identification and characterization of human MUS81-MMS4 structure-specific endonuclease. J Biol Chem. 2003 Jun 13;278(24):21715-20. [Content Brief]
[4]. Ciccia A, et al. Identification and characterization of the human mus81-eme1 endonuclease. J Biol Chem. 2003 Jul 4;278(27):25172-8. [Content Brief]
[5]. Pepe A, et al. Substrate specificity of the MUS81-EME2 structure selective endonuclease. Nucleic Acids Res. 2014 Apr;42(6):3833-45. [Content Brief]
[6]. Gwon GH, et al. Crystal structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates. EMBO J. 2014 May 2;33(9):1061-72. [Content Brief]
[7]. Pepe A, et al. MUS81-EME2 promotes replication fork restart. Cell Rep. 2014 May 22;7(4):1048-55. [Content Brief]
[8]. Hua Z, et al. Crystal structure of the human MUS81-EME2 complex. Structure. 2022 May 5;30(5):743-752.e3. [Content Brief]
[9]. Collie GW, et al. Fragment-Based Discovery of Novel MUS81 Inhibitors. ACS Med Chem Lett. 2024 Jul 11;15(7):1151-1158. [Content Brief]
[10]. Blais V, et al. RNA interference inhibition of Mus81 reduces mitotic recombination in human cells. Mol Biol Cell. 2004 Feb;15(2):552-62. [Content Brief]
[11]. Zhang R, et al. BLM helicase facilitates Mus81 endonuclease activity in human cells. Cancer Res. 2005 Apr 1;65(7):2526-31. [Content Brief]
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