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  4. Histone H4 (Mono Methyl Lys20) Antibody (YA7470)

Histone H4 (Mono Methyl Lys20) Antibody (YA7470)

Art. -Nr.: HY-P87785
COA User Guide for Antibodies Technical Support

Histone H4 (Mono Methyl Lys20) Antibody (YA7470) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H4 (Mono Methyl Lys20).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Background

  • Beschreibung

Beschreibung

Histone H4 (Mono Methyl Lys20) Antibody (YA7470) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Histone H4 (Mono Methyl Lys20).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molekulargewicht
Predicted band size: 11 kDa;
Observed band size: 11 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:500-1:2000
WB
WB: Western Blot
1:2000-1:10000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:5000-1:20000
Sensitivity Endogenous Reinheit Protein A affinity purified
Conjugation Non-conjugated Modification Methylated
Isotype IgG,Kappa  
Appearance

Liquid

Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background
Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
Subcellular Localization:Nucleus,Chromosome
Isoforms & Post-Translational Modification:P62805: 103 amino acids, molecular weight 11367 Da.
Subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers
Dokumentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Produktname:
Histone H4 (Mono Methyl Lys20) Antibody (YA7470)
Art. -Nr.:
HY-P87785
Menge:
MCE Japan Authorized Agent: